tear lactoferrin

LF is named for its ability to be first found in cow's milk and has a strong ability to bind high iron. It is a glycoprotein with an isoelectric point of about pH 7.8 and a molecular weight of 75 to 86 kD. The protein portion consists of a polypeptide chain. LF can inhibit the growth of a variety of bacteria, and can also promote the antibacterial effect of lysozyme, synergistic with SIgA, the antibacterial activity is stronger than when both exist alone. LF is present in various body fluids and secretions such as milk, tears, saliva, semen, urine, pancreatic juice, bile and respiratory secretions. LF in various body fluids of the human body has the same immunological properties. Basic Information Specialist Category: Ophthalmology Classification: Biochemical examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Dry keratitis. Normal value: Tears lactoferrin: 1.04-2.23g/L Above normal: Rare. negative: Positive: Tips: Nutritional intake should be balanced: not partial eclipse, pay special attention to the intake of vitamin B (germ rice, oatmeal yeast). Normal value 1.04 ~ 2.23g / L. After the age of 40, it gradually decreased, and those over 70 years old were 0.85 to 1.63 g/L. Reflective tears have a higher tear content than normal adults >3g/L. Clinical significance Same as lysozyme, but more sensitive. Reflecting tear LF <1g / L has a definite diagnosis value for dry keratitis. Low results may be diseases: dry keratoconjunctivitis, dry eye precautions Inappropriate people: generally no special population. Contraindications before the test: Nutritional intake should be balanced: not partial eclipse, pay special attention to the intake of vitamin B (germ rice, oat yeast). Requirements for inspection: (1) When the transmittance is turbid, the wavelength at which the particles absorb most strongly should be used, otherwise the slope of the curve will be affected. (2) The good performance of the testing instrument should be guaranteed. When measuring the optical system of the instrument, the curve of the turbidimetric method should be redrawn, otherwise it will cause errors. (3) Turbidimetry is susceptible to external conditions. Therefore, the test conditions, reagent concentration, dosage, etc. are consistent. The particle size in the suspension should be uniform and reproducible, otherwise the results will be affected. Inspection process To determine the tear LF, various immunochemical methods can be used, and the immunodiffusion method and the immunoturbidimetric method are usually used in the laboratory, and the two methods are simple and easy. Other LF assays such as ELISA or RIA methods can be performed. McCollum reports that LF can be accurately and quickly measured using a dedicated LF assay card, which takes only 10 to 15 minutes to make routine testing possible. Not suitable for the crowd no. Adverse reactions and risks no.

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