tear epidermal growth factor

Studies have shown that EGF has obvious biological functions, can promote cell mitosis, promote glycolysis and synthesis of DNA, RNA and protein. EGF in tears can stimulate the proliferation of conjunctival epithelial cells and shorten the coverage time of corneal epithelial defects. Excimer laser photorefractive keratectomy (PRK) for the treatment of myopia and tears after radial keratotomy. The EGF content increased significantly and gradually returned to normal, presumably because the elevated tear EGF promoted the healing of corneal wounds. Basic Information Specialist Category: Ophthalmology Classification: Biochemical examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Rare. Normal value: Tear epidermal growth factor: 0.75-7.1ng/ml Above normal: Elevated tear EGF promotes healing of corneal wounds during corneal injury. negative: Positive: Tips: Do not wear clothes that are too small or too tight, so as to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood drawing, causing blood vessels in the arms. Normal value 0.75 ~ 7.1 ng / ml. Clinical significance Abnormal results EGF can promote the regeneration of corneal epithelium after corneal alkali burn, but can not prevent the corneal epithelium from repeated exfoliation secondary to corneal epithelial erosion. Excimer laser photorefractive keratectomy (PRK) for the treatment of myopia and tears after radial keratotomy. The EGF content increased significantly and gradually returned to normal, presumably because the elevated tear EGF promoted the healing of corneal wounds. Need to check the population of corneal damage in the population. High results may be diseases: corneal abrasions precautions Inappropriate people: generally no special population. Taboo before the examination: It is necessary to cooperate with the doctor to write the correct name, neat and tidy, to avoid confusion caused by the same name or similar names. With these in mind, blood draws are more convenient and faster, and you can better save yourself time for diagnosis. Requirements for inspection: Do not wear clothes that are too small or too tight in cuffs to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood drawing, causing blood vessels in the arms. Different laboratory items should be asked by the doctor and treated differently. Inspection process The main reagent components in the ELISA, such as antigens, antibodies, enzyme conjugates, substrates, etc., are easily degraded and inactivated if not properly prepared or improperly stored. There are many steps in the ELISA, and if the operation is not standardized, it is difficult to obtain accurate results. Therefore, in order to ensure the effectiveness of the test, quality control must be performed for each test. The positive and negative controls provided in the excellent kits can generally be used as the quality control of the test. According to the instructions, the effectiveness of the test can be judged from the obtained absorbance values. Taking the most commonly used HBsAg test in clinical tests as an example, the negative control in the kit should be HBsAg-negative human serum, and the positive control should indicate HBsAg content (eg 9±2 ng/ml). Three negative controls and two positive controls should be made simultaneously for each batch of tests. The absorbance value obtained by the negative control assay should be less than a certain value (for example, 0.100), and the mean value of the positive control absorbance minus the negative control absorbance should be a certain value (for example, 0.200). These values ​​are specific experimental values ​​for the kit under the specified assay conditions. Therefore, if the test results meet the requirements, it indicates both the effectiveness of the reagents used and the correctness of the operation. Only in this case, the batch test is effective. Some of the positive and negative controls contained in the kit do not meet the requirements for quality control, or the laboratory's measurement conditions such as colorimeter, etc., and the difference in the kit description, the appropriate quality should be used according to the actual situation. Controls and quality control values ​​from the laboratory derived from the experiment. The quality control serum of the commodity is expensive. Qualitative determination of ELISA quality control products can generally be prepared by themselves. The HBsAg test is still taken as an example. Negative controls can be taken from blood donors who are HBsAg negative with high quality reagents. The positive control can be prepared by adding an appropriate amount of HBsAg-positive serum to the negative control serum, and comparing the mixed serum with a reference standard or a quantitative control to determine the HBsAg content. Then, appropriate dilution is carried out to obtain the HBsAg-positive serum of the desired concentration, and then an appropriate amount of antibacterial preservatives such as gentamicin and salicylic acid are added, and then cryopreserved at a low temperature. Not suitable for the crowd no. Adverse reactions and risks no.

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