tear lysozyme

In the various secretions of human body, the content of lysozyme in tears is the highest, which is more than 1000 times of the serum content. It is mainly produced by lacrimal gland tissue and plays an important role in the local non-specific immunity. Lysozyme is a 14700 molecular weight sequestration protein that catalyzes the hydrolysis of bacterial cell walls. Therefore, it plays an important role in the fight against bacterial infections, especially Gram-positive bacteria. Lysozyme has a very high concentration in tears, accounting for 20-40% of total tear protein, 1000 times higher than serum concentration. It is an important defense substance on the surface of the eye. Basic Information Specialist Category: Ophthalmology Classification: Biochemical examination Applicable gender: whether men and women apply fasting: not fasting Tips: In case of eye disease, you should treat it in time, and be careful not to transmit the bacteria to others. Normal value 1.4 ± 0.5 g / L. Clinical significance Abnormal results: the content of lysozyme and transforming growth factor β2 (transformation growth factor-β2, TGF-β2) in dry eye patients and normal people provides a more valuable basis for the diagnosis of clinical dry eye. Studies have shown that a variety of diseases can change the content of tear lysozyme, but the most indistinguishable problem is that the normal human unstimulated tears and the stimulating reflex tear secretion lysozyme are the most unresolved. Need to check the crowd: people often feel dry eyes, eyes up, eye fatigue. Precautions Inappropriate people: generally no special population. Taboo when checking: In case of eye disease, it should be treated promptly, and be careful not to infect the bacteria to others. Requirements for inspection: 1. When the transmittance is turbid, the wavelength with the strongest absorption of the particles should be used, otherwise the slope of the curve will be affected. 2. The good performance of the test instrument should be guaranteed. When measuring the optical system of the instrument, the curve of the turbidimetric method should be redrawn, otherwise it will cause errors. 3, turbidimetric method is susceptible to external conditions, therefore, the test conditions, reagent concentration, dosage and other requirements are consistent, the particle size in the suspension should be uniform, reproducible, otherwise it will affect the results. Inspection process Methods for measuring lysozyme include immunological methods and non-immunological methods. The non-immunological method uses M. Lysodeikticus as a substrate and is measured by a plate method or a photoelectric nephelometry. This type of method is easy to operate and its detection sensitivity can meet the requirements of tear specimens. At present, the transmittance turbidimetric method is commonly used. Not suitable for the crowd no. Adverse reactions and risks no.

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