von Willebrand factor antigen (vWF:,Ag)

Vascular von Willebrand Factor plays an important role in hemostasis, and it acts as a carrier of Factor VIII, in addition to mediating platelet adhesion to damaged blood vessels. Basic Information Specialist classification: inspection classification: immune examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Reducing vascular hemophilia is an important basis for diagnosis and classification. Normal value: Rocket electrophoresis: 61.63%-123.55% U/ml Enzyme-linked immunosorbent assay: 0.49-1.58U/ml Above normal: Elevation is seen in strenuous exercise, mid-late pregnancy, myocardial infarction, angina pectoris, cerebrovascular disease, kidney disease, liver disease, diabetes, pregnancy-induced hypertension syndrome, major surgery and peripheral vascular disease. negative: Positive: Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. After 8 pm on the day before the medical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. When you draw blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Normal value a) rocket electrophoresis method 94.09% ± 32.46%; b) Enzyme-linked immunosorbent assay 1.02 ± 0.56 U / ml. Clinical significance The von Willebrand Factor antigen assay is mainly used for the diagnosis and differential diagnosis of von Willebrand disease. 1, elevated in: strenuous exercise, late pregnancy, myocardial infarction, angina pectoris, cerebrovascular disease, kidney disease, liver disease, diabetes, pregnancy-induced hypertension syndrome, major surgery and peripheral vascular disease. 2, reduced in: vascular hemophilia, is an important basis for diagnosis and classification. Low results may be diseases: vascular hemophilia, acquired platelet dysfunction, platelet-type pseudovascular hemophilia, high results may be diseases: myocardial infarction, vascular hemophilia precautions 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Inspection process 1) Take a certain amount of VWF:Ag antiserum, dilute 10-fold with Tris-barbital buffer, and then use 5g/L agarose tris-barbital buffer to melt water. , cooled to 56 ° C. Then, the diluted VWF:Ag antiserum pre-warmed to 56 ° C was mixed 1:60 (depending on the antibody titer) to prepare an agar plate of 8 cm × 8 cm and a thickness of 1.5 mm, and a row of about 2 cm from the side of the glass plate. Small hole, diameter hole 3mm, hole spacing is 5mm. (2) Place the agar plate in the electrophoresis tank, the hole toward the cathode, use the filter paper as a salt bridge, and use a quantitative syringe to absorb the healthy human diluted with different ratios of 1:2, 1:4, 1:8, 1:16, etc. 10 μl of each plasma was added to the wells of the antibody agar plate. The plasma samples to be tested were diluted 1 time with Tris-Barbital buffer and added to each sample well. The amount per well was 10 μl. (3) Electrophoresis conditions: electrophoresis 110 ~ 150V, current about 5mA / plate, time 18h, temperature below 15 °C. (4) After electrophoresis is completed, the gel plate is taken out and immersed in 4.4mmol/L phosphomolybdic acid dyeing solution for more than 20min, and then soaked in physiological saline for 10min, the obvious rocket-like precipitation peak can be seen, and the amino black can also be used as usual. dyeing. (5) After staining, measure the height of the rocket electrophoresis peak with double gauge (from the upper edge of the sample well to the peak), and take 5 readings of standard plasma, and obtain a standard curve by regression equation, and then find each sample VWF. The content of Ag is multiplied by the dilution factor (×2), which is the actual concentration of the sample. Indicates the percentage equivalent to normal plasma. Not suitable for the crowd There is no inappropriate crowd. Adverse reactions and risks It is a safe check and is harmless to the body.

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