Phagocytosis chicken erythrocyte function test
Defects in phagocytic function are found in actin insufficiency, membrane glycoprotein deficiency, G-6-PD deficiency, chronic granuloma, malignant tumors, and the like. The macrophage phagocytic function test is mainly used to judge the therapeutic effect of the tumor. The percentage of patients with non-malignant tumors was (67.46±1.32)%, the phagocytosis index was 1.276±0.062, the percentage of phagocytosis in cancer patients was (38.67±1.94)%, and the index was 0.618±0.034. It has been reported that the percentage of phagocytosis in patients with esophageal cancer, gastric cancer, colon cancer, breast cancer, cervical cancer and other malignant tumors is below 45%. The phagocytic function of tumors after surgical resection can be increased to varying degrees, so this test is often used to determine the reference indicators of tumor treatment. Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: The percentage of phagocytosis in cancer patients was (38.67 ± 1.94)%, and the index was 0.618 ± 0.034. The percentage of phagocytosis in patients with esophageal cancer, gastric cancer, colon cancer, breast cancer, cervical cancer and other malignant tumors is below 45%. Normal value: Percentage of phagocytosis: 61.39-64.15% Phagocytosis index: 1.009-1.107 Above normal: The percentage of patients with non-malignant tumors was (67.46±1.32)%, and the phagocytic index was 1.276±0.062. negative: Positive: Tips: Individuals' blister fluids can be coagulated due to the high protein content. After the blister fluid is placed, anticoagulants can be added to the centrifuge tube. Normal value The percentage of phagocytosis in normal subjects was (62.77±1.38)%, and the phagocytic index was 1.058±0.049. Clinical significance The percentage of patients with non-malignant tumors was (67.46±1.32)%, the phagocytosis index was 1.276±0.062, the percentage of phagocytosis in cancer patients was (38.67±1.94)%, and the index was 0.618±0.034. It has been reported that the percentage of phagocytosis in patients with esophageal cancer, gastric cancer, colon cancer, breast cancer, cervical cancer and other malignant tumors is below 45%. The phagocytic function of tumors after surgical resection can be increased to varying degrees, so this test is often used to determine the reference indicators of tumor treatment. Low results may be diseases: esophageal cancer, cervical cancer, stomach cancer, intestinal cancer precautions (1) The fluid volume of different individual blister is different due to different immune status of the body. Healthy people and young people respond strongly, and the amount of blister fluid is high. The course of the disease is long, the condition is heavy and the elderly are weak, the reaction is light, the amount of blister fluid is small, and sometimes it is not blistering. (2) The amount of spotted ethanol leaching solution should be appropriate and constant. The amount of use was high and the reaction was strong. The neutrophils in the 48h epidermis accounted for the majority. A proper amount of macrophages can be obtained in vesicles for 48 hours. (3) The ability of macrophages to digest chicken red blood cells is divided into three levels: 1 undigested: the chicken red blood cell core is clear and the coloration is normal; 2 mild digestion: chicken red blood cell nucleus, nuclear swelling, staining light; 3 complete digestion: chicken red blood cell nucleus, staining is extremely light. Inspection process (1) Take 1ml of blister fluid and add 5% chicken red cell suspension 0.04ml, mix in a siliconized centrifuge tube, transfer to a 37 °C incubator for 30 min, shake once every 10 min. (2) Centrifugation at 1000 r/min for 10 min, take a cell pellet suspension and make a thin slice, dry it, and stain it with Giemsa-Rayleigh. Not suitable for the crowd There is no inappropriate crowd. Adverse reactions and risks It is a safe check and is harmless to the body.
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