Toxicity test in mice

The virulence test of mice is to use a pathogen or a vaccine to infect mice. According to the survival rate and survival time of mice, whether a pathogen or a vaccine is virulence and virulence is identified. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Clinical significance Abnormal results: The mice suffer from various diseases and even death. For example, Streptococcus mutans caused by Streptococcus causes various purulent inflammations, scarlet fever, rheumatic fever and other diseases. Streptococcus pneumonia causes diseases such as lobar pneumonia. People who need to be examined: patients infected with pathogenic microorganisms of unknown virulence, or infections that are not treated with ordinary antibacterial drugs. Positive results may be diseases: suppurative mumps, rheumatic fever, scarlet fever, lobar pneumonia, acute suppurative tenosynovitis considerations Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process For example: the virulence test of mice with freeze-dried live Japanese encephalitis vaccine (1) Pathogenicity test in the brain The production of the poisonous batch was inoculated with 10 mice weighing 12 to 14 g, and each brain was injected with 0.03 ml. Those who died within 3 days after inoculation were not counted, and should be observed for 14 days. If there is a small white rat after 3 days, the pathogenicity should be determined by killing the brain. The virulence in the brain of the mouse should not exceed 3.0 LogLD50/0.03ml, and the mice are injected subcutaneously with 0.1ml10-1 diseased rat brain suspension. Subcutaneous pathogenicity test, observed for 14 days, should be healthy. (2) Subcutaneous infection in human brain test The production of the poisonous batch was inoculated into 10 mice of 10-12 g body weight, each subcutaneous injection of 0.1 ml, while the right side of the brain was empty, observed for 14 days, should be healthy. According to the results, the virulence of freeze-dried Japanese encephalitis live vaccine was evaluated. Not suitable for the crowd Inappropriate crowd: No. Adverse reactions and risks It is a safe check and is harmless to the body.

The material in this site is intended to be of general informational use and is not intended to constitute medical advice, probable diagnosis, or recommended treatments.