Whole blood lactate determination

Lactic acid is a metabolite of anaerobic oxidation (glycolysis) of sugars. Lactic acid is produced in bones, muscles, brain and red blood cells. Excreted by the kidneys after metabolism by the liver. Blood lactate determination can reflect tissue oxygen supply and metabolic status as well as insufficient perfusion. The increase of lactic acid level can be seen in a variety of clinical diseases. Lactic acid determination plays an important role in guiding the treatment of intensive care patients, especially the treatment of myocardial infarction, cardiac insufficiency, and hypoxia caused by insufficient blood flow. Therefore, it is required to submit a diagnostically valuable test result within 30 minutes (including blood draw) by rapid response. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value Whole blood lactic acid 0.5-1.7mmol / L (5-15mg / dl). Clinical significance Abnormal result The result of the test is lactic acid excess Assessment of shock status Elevated blood lactate is common in patients with circulatory shock. Cyclic shock (severe hypotension) can be reduced by blood volume (blood loss or dehydration), decreased cardiac output, or sepsis (septic shock) blood flow disorder. cause. Coronary shunt Intra-aortic balloon pacing Intra-aortic balloon pacing (IABP) is sometimes used as a treatment in difficult heart surgery. Even so, the mortality rate is quite high. If an early indicator suggests that the IABP is unsuccessful, consider other mechanical cardiovascular treatment options. Recent studies have shown that elevated blood lactate can be used as an early indicator of poor IABP reactivity. Classification and treatment of patients with chest disease in emergency department (ED) Elevated blood lactate can be used as an important differential diagnosis indicator for patients with ED due to chest pain, and differential diagnosis of severe heart disease, such as acute myocardial infarction (AMI), multiple congestive heart failure or severe arrhythmia. Lactic acid levels are significantly associated with increased mortality and suggest urgent medical treatment. Patients with normal lactic acid visits can basically rule out the occurrence of AMI. People who need to be examined shock patients, emergency patients. High results may be diseases: precautions for traumatic shock Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Sampling time is very important when measuring blood lactic acid. Quiet value should be sampled quietly before getting up in the morning; blood lactic acid should be determined according to different sports after exercise. Generally, exercise with lower exercise intensity is sampled about 20s after exercise, medium intensity. The exercise was sampled 1 to 6 minutes after exercise, and the high intensity exercise was sampled 3 to 12 minutes after exercise. In the actual test, you can select several times of sampling time (such as 1, 3, 5 ... minutes) sampling. Inspection process Weigh accurately 1g of test sample, precision water 50mL, then add 25mL of sodium hydroxide titration solution (1mol / L), boil for 5 minutes, add 2 drops of phenolphthalein indicator solution, and use sulfuric acid titration solution (0.5mol / L) Titration, until the solution is pink, and the results of the titration are corrected by a blank test, and the volume (mL) of the sulfuric acid titration solution is recorded. Each 1 mL of sodium hydroxide titration solution (0.1 mol/L) is equivalent to 90.08 mg of lactic acid (C3H6O3). ). Not suitable for the crowd No taboos. Adverse reactions and risks No complications.

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