Urine ammonia nitrogen

Most of the ammonia in the human body synthesizes urea through the ornithine cycle in the liver. A part is used for the amination of keto acid, synthesizes glutamine, forms an amine salt in the kidney, and is discharged from the urine. Urinary ammonia nitrogen plays an important role in the diagnosis of diabetic ketoacidosis and liver dysfunction. Basic Information Specialist Category: Inspection Category: Urine/Kidney Function Test Applicable gender: whether men and women apply fasting: fasting Tips: When measuring urine ammonia nitrogen, preservatives must be added. Otherwise, urea in the urine is decomposed by bacteria, and ammonia is released to increase the result. Normal value 52 μmol/d. Clinical significance Urinary ammonia is produced by renal tubular epithelial cells, and ammonia in urine is mainly excreted in the form of ammonium salts, which is one of the important functions for regulating electrolyte balance. In diabetic acidosis, urinary ammonia excretion is significantly increased (urine ammonia does not increase during renal acidosis), pregnancy vomiting, eating acidic foods, and increased urinary ammonia in liver dysfunction. In the case of alkalosis, the amount of urinary ammonia excretion is reduced. Low results may be diseases: high metabolic alkalosis results may be diseases: Diabetes lactic acidosis precautions (1) When measuring urine ammonia nitrogen, preservatives must be added. Otherwise, urea in the urine is decomposed by bacteria, and ammonia is released to increase the result. (2) When the concentration of ammonia in the urine is high, the measurement bottle is liable to cause turbidity, and the amount of the sample can be reduced. (3) Generally, 60-mesh artificial pumice is used, washed with 100g/L sodium hydroxide solution before use, then washed with water to remove residual alkali, then washed with 2% glacial acetic acid, finally washed with ammonia-free water, dried at room temperature, and set aside. (4) If the urine is alkaline, an appropriate amount of glacial acetic acid may be added to the neutral or slightly acid to facilitate the adsorption of ammonia by the artificial pumice. Inspection process (1) Retaining 24 hours of urine with a few milliliters of xylene and recording the total amount of urine. (2) Take a 100ml volumetric flask, add 1g of artificial pumice, add 5ml of distilled water and 1ml of mixed urine for 24h, shake for about 5min. (3) Using distilled water about 25-40 ml, the artificial pumice attached to the wall of the measuring bottle is washed down to the bottom of the bottle, and left to stand for a while, and the liquid in the measuring bottle is slowly discarded. (4) Wash the artificial pumice in the bottle again with distilled water for 2 to 3 times, and carefully pour the washing liquid. (5) Add about 60 to 70 ml of water, and mix 2.5 ml of 100 g/L sodium hydroxide. (6) Take another 100ml volumetric flask, add ammonium sulfate application standard solution (7mmol/L ammonia) 10ml and 100g/L sodium hydroxide 2.5ml, and add distilled water 60~70ml. (7) Add 5 ml of Nessler's reagent to each of the two bottles, add distilled water to 100 ml each, and mix. (8) Colorimetric with 440 nm or blue filter, absorb the absorbance to "0" with water, and read the absorbance of each tube. Not suitable for the crowd Generally no taboos. Adverse reactions and risks No.

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