Urinary N'-methylnicotinamide

Vitamin PP, also known as anti-skin disease vitamins, includes niacin (nicotinic acid, nicotine) and nicotinamide (nicotinamide, nikamide), which can be mutually altered. They exert physiological effects in the body as a coenzyme form of NAD+ (Coenzyme I) or NAP+ (Coenzyme II). Normal people do not contain niacin in their urine and only contain trace amounts of nicotinamide. The main excretion in the urine is the metabolites of nicotinamide, N-methylnicotinamide and N-methyl-2-pyridone-5-amide. Determination of the excretion of N-methylnicotinamide in urine provides insight into the nutritional status of niacin and nicotinamide in the body. Basic Information Specialist classification: growth and development check classification: urine / kidney function test Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in patients with ecdysis. Normal value: Urine N'-methylnicotinamide: 3-17mg/d Above normal: No relevant information. negative: Positive: Tips: Collect 24h urine, dilute 5 to 10 times. The N-methylnicotinamide was added in an amount of 0.5 to 1.2 μg/ml per ml of urine sample for further measurement. Normal value Fluorescence assay 3 ~ 17mg / d. Clinical significance (1) A healthy person receives a suitable diet, and the average N-methylnicotinamide discharge in 24 hours is about 7 mg. It has been observed that the daily urine N-methylnicotinamide excretion in patients with ecdysis is 0.5-0.6 mg. (2) Some people think that the excretion of N-methylnicotinamide in urine per hour on an empty stomach is less than 0.03 mg, or that the excretion of N-methylnicotinamide per 100 ml of urine per hour on an empty stomach is less than 95 μg. The content of nicotinamide in the tissue is lower than normal. (3) It is preferable to carry out a saturation test by determining the nutritional status of nicotinamide by oral administration of 50 mg of nicotinamide. If the excretion of N-methylnicotinamide in the urine is less than 0.5 mg every 4 hours, it means that nicotinamide is deficient. Low results may be diseases: niacin deficiency in children, niacin deficiency precautions Collect 24h urine and dilute 5 to 10 times. The N-methylnicotinamide was added in an amount of 0.5 to 1.2 μg/ml per ml of urine sample for further measurement. Inspection process (1) Take 10 ml stoppered test tubes and indicate the measuring tube (U) and standard tube (S). 2 ml of urine and 0.2 ml of glacial acetic acid were added to the tube, and diluted to 10 ml with water. A standard solution of 10 ml of N'methylnicotinamide was added to the standard tube. Add 100 mg of activated carbon to each of the two tubes, mix them several times, immediately filter with filter paper, and take the filtrate for determination. (2) Take 10 tubes of 10 ml stoppered tubes, indicate the measuring tube (U), and measure blank tube (UB), standard tube (S) and standard blank tube (SB). (3) Fluorescence measurement: first read the reading to 50-80 with quinine sulfate application solution. The excitation wavelength is 380-360 nm, the emission wavelength is 430-450 nm, and the fluorescence intensity of each tube is read. Not suitable for the crowd Generally no taboos. Adverse reactions and risks No.

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