Urinary 17-ketosteroids

17-ketosteroide (17-KS) refers to those with corticosteroids and sex hormones having a ketone group at the 17-carbon position, including androsterone, epiandrosterone, dehydroepiandrosterone, 1 1-oxo-androstenone, 11 - Hydroxy androstenone, etc., mostly in a combined form. 17-ketosteroids are mainly metabolites of the adrenal gland and testicular androgen. Two-thirds of men are from the adrenal gland, one-third are from the testis, and women are mainly from the adrenal gland. The general condition of urinary 17-ketosteroid (17-KS) in response to adrenocortical hormone, glucocorticoids and gonad secretion is of great value for evaluating the function of the adrenal gland to secrete androgen. Basic Information Specialist classification: urinary examination classification: urine / kidney function test Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Adrenal insufficiency, hypopituitarism, testicular dysfunction, cirrhosis, and chronic wasting diseases such as diabetes, tuberculosis, and high malnutrition, urine 17-KS can be reduced. Normal value: Urine 17-ketosteroids (male): 8-12mg/24h Urine 17-ketosteroid (female): 6-9mg/24h Above normal: Urinary 17-KS was significantly increased in Cushing's syndrome, testicular tumors (especially stromal cell tumors), precocious puberty, polycystic ovary, and acromegaly. Urine 17-KS can also be increased by the use of drugs such as androgen, corticosteroids and ACTH. negative: Positive: Tips: Anhydrous ethanol must be de-aldehyde treated. If the room temperature is too low, the colorimetric liquid may be turbid. It should be added with 0.1ml of saturated brine before the turbidity disappears before colorimetry. If the urine cannot be measured in time, it should be stored in the refrigerator to avoid the destruction of 17-ketosteroid. The result is reduced. Normal value Male 8 ~ 12mg / 24h urine; female 6 ~ 9mg / 24h urine. Clinical significance (1) The urinary 17-KS of Cushing's syndrome, testicular tumor (especially stromal cell tumor), precocious puberty, polycystic ovary, acromegaly and other diseases were significantly increased. Urine 17-KS can also be increased by the use of drugs such as androgen, corticosteroids and ACTH. (2) Adrenal insufficiency, hypopituitarism, testicular dysfunction, cirrhosis and chronic wasting diseases such as diabetes, tuberculosis, and high malnutrition, and urinary 17-KS may be reduced. Low results may be diseases: acromegaly, high cirrhosis results may be diseases: polycystic ovary syndrome, adrenal insufficiency, precocious puberty (1) The anhydrous ethanol used must be subjected to dealdehyde treatment, otherwise the blank tube reading is too high, which affects the accuracy of the results. (2) Moisture can affect the condensation of m-dinitrobenzene with 17-ketosteroids. Therefore, after evaporation of ether, no water should remain in the tube. (3) The color development of this method is not stable enough, the colorimetric should be completed within 10 minutes, and a large number of specimens should be colored in batches. (4) If the room temperature is too low, the colorimetric liquid may be turbid. It should be added with 0.1 ml of saturated brine before the turbidity disappears before colorimetry. (5) If the urine cannot be measured in time, it should be stored in the refrigerator to avoid the destruction of 17-ketosteroids and the result is reduced. Inspection process (1) Concentrated hydrochloric acid 5 ml was added as a preservative in a urine container. Collect 24h urine, mix, and measure the total amount. (2) Take 5ml of urine sample, put it into a 20mm × 150mm test tube, add 15ml of concentrated hydrochloric acid; boil in boiling water bath for 20min, take it out, and cool it in cold water. (3) Cool the urine sample into a 30 ml small separatory funnel, add 10 ml of ether, shake for 2 min, place it to be layered and discard the lower layer of urine. (4) Add 5 ml of 1 mol/L sodium hydroxide to the small funnel, shake gently for 1 min, wash the ether, set to clarify, and discard the lower aqueous phase. (5) Re-use 2.5 ml of distilled water, gently shake the ether for 1 min, set to clarify, and discard the lower aqueous phase. (6) The ether was transferred to a 15 ml test tube and evaporated to dryness in a water bath at 40 to 45 ° C. This tube was the measuring tube. (7) Set the measuring tube, standard tube (containing male ketone standard 0.01mg), blank tube, mix each tube, and centrifuge at 1000r/min for 2min, and transfer the upper layer solution into the 10mm optical diameter comparison cup. The wavelength of 520 nm is zeroed by blank, and the absorbance of each tube is read. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally not.

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