Urinary 17-hydroxycorticosteroids

After cortisol secreted by the adrenal cortex is inactivated by the liver, most of them are in the form of glucuronic acid ester or sulfate. They are collectively called 17-hydroxycorticosteroids, which are excreted by urine, and the daily discharge accounts for 30% to 70% of the total. . Urinary 17-hydroxycorticosteroid is a hormone secreted by the adrenal cortex in the urine and its metabolites, mainly cortisol, tetrahydrocortisol, cortisol, tetrahydrocortisol and the like. Urinary 17-hydroxycorticosteroid content can reflect the secretion of cortisol from the adrenal cortex and contribute to the diagnosis of certain endocrine diseases. Leave 24h urine to calculate the total amount (with 5 to 10ml of concentrated hydrochloric acid), take 10ml for inspection. Basic Information Specialist classification: growth and development check classification: urine / kidney function test Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in primary or secondary adrenal insufficiency, hypopituitarism or bilateral adrenalectomy, malnutrition, cirrhosis, tuberculosis and some chronic wasting diseases. Normal value: Urine 17-hydroxycorticosteroid (male): 5-15mg/24h Urine 17-hydroxycorticosteroid (female): 4-10mg/24h Above normal: Found in adrenal hyperfunction (caused by tumor or hyperplasia), ectopic ACTH syndrome, surgery, obesity, hyperthyroidism, acute disease or a large number of hormones (cortisone) treatment, pancreatitis. negative: Positive: Tips: In order to prevent the interference of plant pigments on urine samples, stop taking Chinese herbal medicines or pigmented drugs before the test, and stop drinking tea for more than 1 week. Normal value Male 13.8 ~ 41.4μmol / 24h (5 ~ 15mg / 24h). Female 11 ~ 27.6μmol / 24h (4 ~ 10mg / 24h). Clinical significance Reduced in primary or secondary adrenal insufficiency, hypopituitarism or bilateral adrenalectomy, malnutrition, cirrhosis, tuberculosis and some chronic wasting diseases. Elevation is seen in adrenal hyperfunction (caused by tumor or hyperplasia), ectopic ACTH syndrome, surgery, obesity, hyperthyroidism, acute disease or massive hormone (cortisone) treatment, pancreatitis, etc. Low results may be diseases: secondary adrenal insufficiency, high pancreatitis results may be diseases: pregnancy with chronic adrenal insufficiency, endocrine hypertension, primary adrenal insufficiency In order to prevent the interference of plant pigments on urine samples, stop taking Chinese herbal medicines or pigmented drugs before the test, and stop drinking tea for more than 1 week. Inspection process (1) Collect 24h urine, and pre-concentrate 5~10ml of concentrated hydrochloric acid in the urine bottle to prevent corrosion. (2) Aspirate 5 ml of mixed urine, place it in a stoppered test tube, and adjust the pH to 1 with 620 ml/L of sulfuric acid. (3) Add 1.5 g of anhydrous sodium sulfate, mix immediately, add 5 ml of n-butanol, shake for 5 min, and centrifuge to precipitate. (4) Pipette the upper layer of n-butanol and another tube, add 200-250mg of anhydrous sodium carbonate, shake well, let stand for 5min, remove the interference pigment, centrifuge and precipitate, add and plug, 60 ° C water bath for 20min, After cooling for 5 min, the color was measured with a wavelength of 420 nm, and the tube was zeroed, and the absorbance was read. Not suitable for the crowd Generally no taboos. Adverse reactions and risks No.

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