Schistosoma ring egg sedimentation test

The schistosomiasis egg-precipitation test is mainly used to detect specific antibodies against schistosomiasis in serum. The ring egg precipitation test is mainly used to detect specific antibodies against schistosomiasis in serum. If it is positive, the positive rate of recent infection with schistosomiasis is over 90%, and the positive rate of positive detection with feces is over 95%. It has early diagnostic value for schistosomiasis infection. The systemic treatment of schistosomiasis patients may be negative after 3 to 4 years. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The positive rate of patients was 97.3% (94.1%-100%), the false positive rate was 3.1% (2.5%-5.6%), and the cross-section of schizophrenia and filariasis patients was 0.5%~1%. reaction. The cross-reaction of patients with paragonimiasis was 6.4%. Tips: The stool must be fresh, and the inspection time should not exceed 24h. Normal value The schistosomiasis egg ring sedimentation test was negative. Clinical significance The ring egg precipitation test is mainly used to detect specific antibodies against schistosomiasis in serum. If it is positive, the positive rate of recent infection with schistosomiasis is over 90%, and the positive rate of positive detection with feces is over 95%. It has early diagnostic value for schistosomiasis infection. The systemic treatment of schistosomiasis patients may be negative after 3 to 4 years. The positive rate of patients was 97.3% (94.1%-100%), the false positive rate was 3.1% (2.5%-5.6%), and the cross-section of schizophrenia and filariasis patients was 0.5%~1%. reaction. The cross-reaction of patients with paragonimiasis was 6.4%. After receiving effective treatment, the ring egg reaction intensity gradually decreased. In the epidemic area, the change in the positive rate of the population's ring egg reaction can be used as an evaluation of the therapeutic effect, but the reaction requires a longer time. Positive results may be diseases: schistosomiasis cerebral dermatitis considerations (1) The number of eggs to be added should be appropriate, and too many or too few eggs have a certain effect on the reaction results. When calculating eggs, no immature eggs and broken eggs are counted. (2) For each batch of serum samples, a positive serum control should be performed at the same time. (3) This test can be used for the reaction of blood-dropping filter paper instead of serum, but when the blood droplets are eluted, the intensity of the diluted reaction of the blood is thus lowered. Dry blood filter paper is generally not recommended in China. At present, there are heat-treated ultrasonic dry eggs, PVC film ring egg reaction and double-sided tape strip egg reaction. (4) The stool must be fresh, and the inspection time should not exceed 24 hours. Inspection process (1) Using a molten paraffin, draw two straight lines of about 20 mm on a clean glass slide, and add 2 drops of the serum of the subject between the wax lines. (2) Pick 100-150 dry eggs from the needle tip and add them to the serum, and cover them. Cover the 24mm~24mm coverslips. The coverslips are sealed with paraffin. (3) After 48 hours in a 37 ° C incubator, the reaction results were observed with a low power microscope. Suspected positives should be identified under a high power microscope. (4) Interpretation of the results: A typical positive reaction is the appearance of a bubble-like, finger-like or slender, curled band-like deposit around the eggs, with neat edges and obvious refraction. The vesicular precipitate must be greater than 10 [mu]m (approximately 2 cell sizes). 1 The reaction intensity is represented by (+) to (3+). (3+) The area of ​​vesicular deposits around the eggs is larger than the area of ​​the eggs, and the banded sediments are equivalent to or exceed 2 times the long diameter of the eggs. (2+) The area of ​​the vesicular deposit around the egg is larger than 1/2 of the area of ​​the egg, and the banded precipitate corresponds to or exceeds the long diameter of the egg. (+) The area of ​​the bubble-like precipitate around the egg is less than 1/2 of the area of ​​the egg, and the band-shaped sediment is smaller than the long diameter of the egg. 2 Negative reaction: There is no sediment around the eggs, or there are vesicular precipitates smaller than 10μm and spherical regardless of size (spherical, slightly dilated, non-connected with eggs) and shadow (the shape is not very regular) , the light is light, and the particles are visible under high magnification. For the positive reaction specimens, 100 mature eggs were observed, and the number of eggs in response and the intensity of the reaction were recorded, and the ring-up rate was calculated. Negatives must look at the complete film. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No relevant information.

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