Serum immunoglobulin D (IgD)

Serum immunoglobulin D (IgD) content is very low, can exist as a membrane receptor on the surface of B cells, its role may be involved in the initiation of B cell production of antibodies, its function is also related to certain autoantibodies and anti-toxin antibodies, such as antinuclear Antibodies, anti-basement membrane antibodies, anti-thyroid antibodies, anti-diphtheria toxoid antibodies, tetanus toxoid antibodies, etc. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Normal value ELISA method, RID method (radioimmunoassay method) The newborn is negative. Adults 1 ~ 60mg / L (0.1 ~ 4mg / dl). (Note the specific reference value depends on each laboratory.) Clinical significance (1) rise 1 multiple high IgDemia chronic infectious diseases (tuberculosis, leprosy, osteomyelitis, suppurative skin disease), Kwash-iorkor (Quaic Oak disease, malignant malnutrition), atopic disease, partial primary immunity Defects (high IgMemia, with immunoglobulin deficiency, IgA alone deficiency), periodic fever (2-12 years), etc. 2 simple high IgD hypertensive IgD myeloma, benign simple immunoglobulinemia, IgD multiple myeloma. (2) lower 1IgD-deficient family (autosomal abnormality), IgD, IgA, IgM immunoglobulin reduced to primary immunodeficiency syndrome (transient hypogammaglobulinemia in neonates, gamma-globulinemia in infants) ), severe combined immunodeficiency syndrome (SCID), Good syndrome. 2IgD significantly reduced or even disappeared sarcoma-like disease, and IgD alone lacked a tendency to be susceptible. High results may be diseases: tuberculosis, leprosy, severe combined immunodeficiency disease, cavitary tuberculosis, invasive pulmonary tuberculosis, primary immunodeficiency disease First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process (1) One-way immunodiffusion method: same as IgG determination, but the serum to be tested is not diluted, and two duplicate wells are set for each sample. After the sample is applied, it is spread at 37 ° C for 48 hours, and the IgD reference product is diluted to 10 in the preparation of the standard curve. , 50, 100, 150 and 200 U/ml. (2) ELISA method: according to the kit instructions. Or refer to the steps below. 1 The reaction plate microwells were coated with IgG fraction (1.0 μl/ml) coated with diluted horse anti-human IgD, 100 μl per well. After 4 ° C overnight, wash with washing solution 3 times for 3 min each time (the same below). 2 plus serum to be tested (diluted 1:200 with dilution) 100 μl or various concentrations of IgD reference (2.5, 1.25, 0.625, ..., 0.02 U/ml), 100 μl per well. There are 2 duplicate holes. Wash at 37 ° C for 2 h. 3 plus the optimal concentration of rabbit anti-human IgD serum, 100 μl per well. Wash at 37 ° C for 2 h. 4 Add the optimal concentration of goat anti-rabbit IgD-HRP, conjugate, 100 μl per well. Wash at 37 ° C for 2 h. 5 plus substrate solution, 100 μl per well, 37 ° C for 30 min. The reaction was stopped by adding 2 mol/L H 2 SO 450 μl, and the absorbance was measured at 492 nm. According to the standard curve of absorbance of the sample, the corresponding IgD content (U/ml) was calculated. 1U = 1.41 μg. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No related complications and hazards.

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