Serum complement lytic immune complex activity

In complement CRA, the bypass activation pathway (AP) plays a leading role, and the traditional activation pathway is only a promoting effect. The mechanism of complement CRA is that when complement interacts with IC, AP C3 convertase cleaves C3 in large amounts, and a large amount of C3b is inserted into the lattice structure of the antigen antibody and firmly binds to the antibody, causing the binding bond of some antigen antibodies to break. The larger IC agglomerates become smaller agglomerates and dissolve in the liquid phase. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Complement is not an IC that dissolves all antigen-antibody complexes and only dissolves soluble antigens (polysaccharides, proteins, haptens, etc.). Normal value (1) Enzyme-anti-enzymatic method The normal human CRA (X±SD) is 0.38±0.12, and the normal reference range may be X±2SD, that is, 0.14 to 0.62. (2) Radioimmunoassay The normal dissolution rate of adults was 66.0 ± 7.4%. Clinical significance Involved in autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, chronic active hepatitis, glomerulonephritis, etc., the ability of complement lysis complex is low. Normal complement system prevents the appearance of pathological IC or reduces the inflammatory damage caused by IC activation of complement. The congenital or acquired defects of complement and the activation of the complement system may be important causes of pathological IC. Precautions Enzyme-antibiotic method: (1) Complement is not an IC that dissolves all antigen-antibody complexes and dissolves only soluble antigens (polysaccharides, proteins, haptens, etc.). (2) Other precautions with the enzyme immunology technology section. Inspection process (1) Enzyme-anti-enzyme method: 1 Take 0.2 ml of HRP-anti-HRP complex, add 0.1 ml of serum to be tested, set the centrifuge tube at the tip of the bottom, and immerse in a water bath at 37 ° C for 1 h. 2 Add 1 ml of PBS, 3000 r / min, and centrifuge for 15 min. 3 Take 0.5 ml of the supernatant plus 0.4 ml of the substrate solution, and color at 37 ° C for 1 h. The reaction was stopped by adding 0.1 ml of 1 mol/L NaOH, and 3 ml of PBS was added thereto, and the absorbance at 450 nm was measured. 4 Using PBS 0.1 ml instead of the test serum as a control tube to zero. (2) Radioimmunoassay: 1 test group: A. Dilute the test serum 45 μl with Ca2+, Mg2+ gelatin barbiturate buffer 1:3, 125 IBSA-anti-BSA complex suspension 5 μl at 37 ° C for 15 min and add 1 ml of cold PBS (pH 7.20.15 mol / L). B. Centrifuge at 1800 r/min for 20 min, discard the supernatant, and measure the precipitated cpm with a γ-scintillator. 2 Control group: Naturally dissolved, normal human mixed serum was inactivated at 56 ° C for 30 min to take 45 μl. Normal dissolution: normal humans mix fresh serum and take 45 μl. The other reagents added to the control group were the same as the experimental group. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No related complications and hazards.

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