Serum alternative complement pathway activity

This test blocks the traditional activation pathway of complement, adding rabbit red blood cells to activate factor B, leading to activation of complement bypass, and rabbit red blood cells are damaged and hemolysis occurs. The relationship between hemolysis rate and complement bypass activity is similar to CH50. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: The serum to be tested must be fresh. If left at room temperature for more than 2 hours, the complement activity will decrease. Normal value The normal value of healthy people measured by this method is 50-100u/ml. Clinical significance In many pathological conditions, serum complement levels can be changed, so the clinical changes in the content of complement, such as total complement activity, changes in the individual components of complement, especially C3 and C4, etc., for the diagnosis of certain diseases, etiology and Prognosis has a certain meaning. However, the decrease in the amount of complement is not necessarily an immune disorder or an immune disease. It is known that ischemia, coagulative necrosis and toxic necrosis can release more proteolytic enzymes in tissues, leading to complement hemolytic activity and a decrease in each complement component. Some congenital complement deficiency can also cause a defect in a complement component, so a detailed analysis of the specific situation is required. Precautions (1) The serum to be tested must be fresh. If left at room temperature for more than 2 hours, the complement activity will decrease. (2) The hemolytic activity of complement is affected by many factors, such as sheep red blood cell concentration and the amount of sensitized antibodies. When the antibody molecule adsorbed per uniform sensitive red blood cell is less than 100, the degree of red blood cell solubility decreases as the cell concentration increases. When sensitized with a high concentration of antibodies, the degree of hemolysis increases as the cells increase. A doubling of the red blood cell concentration can increase the amount of 50% hemolysis complement by about 25%. The presence of calcium and magnesium ions stabilizes the hemolysis system. However, when the content is too much, the hemolysis reaction is inhibited. Therefore, in the test, it is necessary to strictly control all aspects of the reaction. (3) This test cannot measure the absolute value of the complement protein, nor can it measure the components in the properdin system. Inspection process Serum cell test. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No related complications and hazards.

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