Treponema pallidum antibody

Syphilis is a sexually transmitted disease caused by infection with P. pallidum. Specific antibodies appear after the pathogen infects the human body. When Treponema pallidum invades the human body, specific antibodies may appear in the serum; in addition, non-specific antibodies (reactants) may also be present in the serum. Detection of Treponema pallidum antibodies is helpful in diagnosis. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Syphilis infection. Tips: If there is local congestion, use a warm towel after 24 hours to promote absorption. Normal value negative. Clinical significance This project is used for the diagnosis of syphilis. Two antibodies appear after infection with syphilis: 1 is a specific brake antibody, which is lgM. When there is complement and anaerobic conditions, it can inhibit the motility of live spiral, and can kill or dissolve the spiral. It has a protective effect on reinfection of the body. 2 The other type is a responsive element, which is a mixture of lgA and lgM, which can non-specifically bind to the lipid antigen in normal biological tissues and has no protective effect on the human body. Precautions Preparation before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection; guests with a history of fainting, please explain in advance, we will make special arrangements. Requirements for inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Not suitable for people: There is no inappropriate crowd. Inspection process method: (1) Double antigen sandwich enzyme-linked immunosorbent assay (ELISA): pre-coated with the Treponema pallidum gene recombinant antigen on the microplate, and then added the enzyme-labeled gene recombinant antigen to specifically bind to the Treponema pallidum antibody in the serum to be tested, Color, the absorbance A value is detected by a microplate reader to determine the trehalose antibody kit and the microplate reader in the sample. (2) Syphilis particle agglutination test (TPPA method): The purified bacterial cell component of syphilis is coated on artificial carrier gelatin particles, and the particles react with the syphilis spirochete in the sample to form agglutination reagent. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.

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