Antibody-dependent cellular (K cell) toxicity

K cells (KillerCell) are a class of lymphocytes that are neither T nor B. It has no surface Ig, but has an Fc receptor and can play the role of killing target cells with the participation of specific antibodies. When the target cell surface antigen binds to the corresponding antibody, the Fc fragment of the antibody recombines to the surface of the K cell to form an antibody bridge to contact the target cell to cause a killing effect, so the K cell is also called an antibody-dependent cytotoxic cell (Antibody-DependentCell- mediatedCytotoxic, ADCC). This killing effect is non-specific and does not require complement participation. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: No clinical significance. Normal value: Normal value: 3-15% Normal value: 3.5-3.5% Above normal: Abnormal immune function. negative: Positive: Tips: Do not take drugs that affect K cytotoxicity before the test. Normal value K cells account for 3% to 15% of the total number of peripheral lymphocytes, and K cells account for 2.5% to 3.5% of peripheral blood. Clinical significance The detection of K cell viability can determine the body's immune function, analyze leukocyte antigens and antibodies, and understand the immune status of the body during organ transplantation. High results may be diseases: Acquired immunodeficiency syndrome considerations Pre-test contraindications: Take medications that affect K-cell toxicity. Requirements for examination: Detection of body K cell activity as an indicator of immune function. Inspection process Methods of Examination: Methods for detecting antibody-dependent cellular cytotoxicity (ADCC) do not require labeling and can be performed on adherent cells in real time. The method includes, for example: (a) monitoring impedance between electrodes on a non-conductive substrate supporting the growth of target cells in a detection medium; (b) adding an effector cell to the detection medium and an antibody binding to the target cell After the addition of effector cells and antibodies, the decrease in impedance between the electrodes on the substrate indicates that ADCC function has been effective in the assay medium. Not suitable for the crowd Inappropriate crowd: None. Adverse reactions and risks No special complications.

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