Anti-Deoxyribonucleic Acid (DNA) Antibodies

Anti-deoxyribonucleic acid (DNA) antibodies are often detected in the serum of various autoimmune diseases, such as systemic lupus erythematosus (SLE), in which anti-single-stranded DNA (ssDNA) antibodies are found in various autoimmune diseases and connective diseases. Tissue is in the blood of the patient, so there is not much diagnostic specificity. However, anti-double-stranded DNA (dsDNA) antibodies are mainly found in SLE patients and are a relatively specific diagnostic indicator. Identification or specific detection of anti-dsDNA antibodies depends on the nature and purity of the dsDNA of the antigen used. Previously, non-pathogenic short-membrane worms and antigens isolated therefrom were used. However, in addition to indirect immunofluorescence, genetic engineering is used to produce dsDNA antigens. , its specificity is better. Hemagglutination test enzyme immunization is a more common method for detecting anti-dsDNA antibodies, but the Farr method is still the standard reference method. Basic Information Specialist classification: inspection classification: immune examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: There is a problem with the immune system. Tips: Because of the need to draw blood, patients need an empty stomach. Normal value Latex agglutination test, convective immunoelectrophoresis, immunofluorescence technique, and enzyme-linked immunostaining were all negative. Clinical significance Abnormal results anti-deoxyribonucleic acid antibody (Anti-DNA antibody) detection, is a highly specific test for the diagnosis of systemic lupus erythematosus (SLE), high titer anti-deoxyribonucleic acid antibody, almost only in patients with systemic lupus erythematosus . In patients with active systemic lupus erythematosus, the positive rate of anti-DNA antibody was 93%, and the positive rate of non-active period was only 7%. Need to check the systemic systemic lupus erythematosus (characterized pathological factors), systemic sclerosis, dermatomyositis, nodular polyarteritis (50% positive), rheumatoid arthritis (10% positive), liver disease, thyroid Inflammation, ulcerative colitis, myasthenia gravis (4% to 17% positive), localized discoid lupus erythematosus (unstable number of positive) and other patients. Positive results may be diseases: systemic lupus erythematosus, elderly rheumatoid arthritis precautions Contraindications before the examination: Due to the need to take blood, the patient needs an empty stomach. Requirements for inspection: It is advisable to use fresh serum samples. If frozen, it can only be frozen once. Inspection process 584 serum samples were detected by immunofluorescence assay using Trypanosoma cruzi. The positive rate of 189 patients with SLE was 52.3%, and 24 patients with active SLE were positive. 77 cases of connective tissue disease, 184 other diseases and 110 healthy controls were negative. The method has a coincidence rate of 96.3% with the immunofluorescence method of the green fly, and it is considered as a substrate specificity test. The author believes that this method has high specificity and sensitivity to the active systemic lupus erythematosus, and the operation is simple and convenient. The advantages of reliability and repeatability can be used as a new method for diagnosing SLE. Not suitable for the crowd Inappropriate people: no special taboos. Adverse reactions and risks There are no related complications.

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