passive hemagglutination assay

Passive hemagglutination is used for the diagnosis of epidemic encephalitis. This method is highly specific, simple and rapid, and does not require special equipment. It is suitable for the majority of primary hospitals. The patient took the cerebrospinal fluid and diluted the antigen with the 96-well V-type hemagglutination plate to dilute the JE antigen, so that each well contained 25 uL, and the serum to be tested was diluted 1:10 with each dilution. Add 25uL, add lyophilized Japanese brain monoclonal antibody to sensitize sheep red blood cells 25uL, and observe the results after standing at 37 °C for several hours. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The serum agglutination titer of the test was 4 times or more than 4 times higher than the antigen control. The serum antigen titer during the recovery period was 4 or more times higher than the acute phase and was positive. Tips: The diet should be regular and reasonable, that is, high-protein, high-vitamin foods. Normal value The result was negative. Clinical significance Abnormal results: The serum agglutination titer of the test was 4 times or more than 4 times higher than the antigen control. The serum antigen titer during the recovery period was 4 or more times higher than the acute phase and was positive. Need to check the crowd: patients with epidemic encephalitis. Precautions Contraindications before inspection: Pay attention to protecting the brain. Taboo when checking: 1, the patient takes the cerebrospinal fluid to sit, so as not to hurt the head. 2, the monoclonal antibody used to sensitize sheep red blood cells to be lyophilized before use. Inspection process The patient took the cerebrospinal fluid and diluted the antigen with the 96-well V-type hemagglutination plate to dilute the JE antigen, so that each well contained 25 uL, and the serum to be tested was diluted 1:10 with each dilution. Add 25uL, add lyophilized Japanese brain monoclonal antibody to sensitize sheep red blood cells 25uL, and observe the results after standing at 37 °C for several hours. Not suitable for the crowd Not suitable for checking the crowd: none. Adverse reactions and risks No related complications or hazards.

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