T lymphocyte subsets

The determination of T lymphocyte subsets is an important indicator for detecting cellular immune function, and it is helpful for the diagnosis of certain diseases (such as autoimmune diseases, immunodeficiency diseases, malignant tumors, blood diseases, allergic diseases, etc.). Mechanism, observation of efficacy and monitoring of prognosis are of great significance. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Tips: Taboo overeating and strenuous exercise, should be calm, fasting blood test. Normal value Immunofluorescence, bridging enzyme immunoassay, SPA wreath method: The positive rate of CD3+ cells was 71.5%±6.2%; The positive rate of CD4+ cells was 45.7%±5.3%; The positive rate of CD8+ cells was 27.9%±5.0%; The CD4/cD8 ratio is 1.66 ± 0.33. Flow Cytometry: The positive rate of CD3+ cells is 61%-85%: The positive rate of CD4+ cells is 28% to 58%: The positive rate of CD8+ cells is 19% to 48%: The CD4/CD8 ratio is 0.9--2.0. Clinical significance Abnormal results: 1. CD4 lymphocyte reduction is seen in patients with malignant tumors, hereditary immunodeficiency disease, AIDS, and immunosuppressive agents. 2. CD8 lymphocytosis is seen in autoimmune diseases such as SLE, chronic active hepatitis, tumors and viral infections. 3. The ratio of CD4/CD8 abnormal AIDS patients was significantly lower, mostly below 0.5. The ratio is also reduced by SLE nephropathy, infectious mononucleosis, acute cytomegalovirus infection, and recovery period of bone marrow transplantation. The increase in the ratio is found in rheumatoid arthritis and type I diabetes. It can also be used to monitor rejection of organ transplants. If CD4/CD8 is significantly increased after transplantation, rejection may occur. The people who need to be examined are: patients with related diseases and those with low immunity can be examined. Low results may be diseases: pediatric acquired immunodeficiency syndrome, early alopecia, pediatric X-linked hyperimmune globulinemia, AIDS, infectious mononucleosis, high results, possible diseases: rheumatoid arthritis Kidney damage, type I diabetes precautions Taboo before the examination: taboo overeating and strenuous exercise, should be calm, fasting blood test. Requirements for inspection: Note that serum samples are not contaminated and sent to the test in time. And follow the doctor's request. Inspection process In the two-dimensional Dot-plot diagram of pre-scattering (FSC) and side-scattering (SSC), the lymphocyte area is drawn, and then the fluorescence intensity of FITC and PE is detected on the lymphocytes. The fluorescence I (FL1) is FITC, filtered. The chip is (530±10) nm bandpass, the fluorescence II (FL2) is PE, and the filter is (575±10) nm bandpass. For data analysis, the limits were determined based on the non-specific fluorescence intensity of isotype to determine double negative, double positive, and single positive regions before determining the percentage of positive cells per test. Not suitable for the crowd Inappropriate people: no special requirements. Adverse reactions and risks There are no related complications and hazards.

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