Agar diffusion test

The agar diffusion test is a precipitation test of a soluble antigen and a corresponding antibody in a semi-solid gel (agar or agarose) containing an electrolyte. In the experiment, the agar only acts as a grid, and the water content is 99%. The soluble antigen and the antibody can diffuse freely. If the antigen corresponds to the antibody, the ratio is appropriate. A white precipitate line can be formed at the meeting, which is a positive reaction. The precipitation line can remain in a fixed position in the gel for a long time and can be dried after dyeing. The precipitation line (band) is specifically impermeable to antigens and antibodies, but not specific. Therefore, a precipitation line (band) represents a precipitate of an antigen and an antibody, and thus the assay can be performed on different antigen-antibody systems in solution. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: It is generally normal. Positive: Prompt chronic or subacute infection, malaria, infectious mononucleosis. Tips: Forbidden overeating and strenuous exercise, suitable for fasting in the morning. Normal value The test result was negative. Clinical significance Abnormal result Increased intrauterine infection, neonatal TORCH syndrome, chronic or subacute infection, malaria, infectious mononucleosis, mycoplasma pneumonia, liver disease, connective tissue disease, macroglobulinemia, asymptomatic monoclonal IgM disease Wait. Reduce hereditary or acquired antibody deficiency, mixed immunodeficiency syndrome, selective IgM deficiency, protein-losing enteropathy, burns, anti-Ig antibody syndrome (mixed cryoglobulinemia), immunosuppressive therapy Wait. People who need to be checked Newborns and patients with related diseases and symptoms should be examined. Positive results may be diseases: viral dermatitis, fungal allergic asthma, brucellosis considerations Taboos during the examination: taboo overeating, and strenuous exercise, suitable for fasting in the morning. Requirements for inspection: Pre-test should be performed before the test to determine the dilution of the antibody. Do not cut the agar when loading, so as not to affect the formation of the sedimentation line. Do not confuse antibodies and antigens at different concentrations when loading, affecting the test results. Inspection process The two-way agar diffusion test is to add soluble antigen and antibody to small holes with a certain distance and pore diameter on a semi-solid agar plate containing electrolyte, so that they can mutually diffuse under certain conditions, and the two meet at a proper ratio to form. White sedimentation line (belt). Multiple precipitation lines can occur when multiple antigen-antibody systems are present, and the antigen-antibody system can then be analyzed based on the number, location, and shape of the precipitation lines. One-way agar diffusion, also known as unidirectional circular immunodiffusion, is a method for quantitative determination of antigen. It is often used to detect the content of various immunoglobulins in serum and various complement components in serum. High sensitivity, can be used as an auxiliary diagnosis for immune diseases. Not suitable for the crowd No taboos. Adverse reactions and risks No complications.

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