Pancreatic carcinoembryonic antigen (POA)

The specificity of pancreatic carcinoembryonic antigen is not high, but it can be used to observe the efficacy of pancreatic cancer resection, an indicator of pancreatic cancer recurrence monitoring. Clinically, radioimmunoassay is commonly used. Increased in pancreatic cancer (positive rate 48% to 75%), acute pancreatitis (positive rate of 80%), chronic pancreatitis (positive rate of 15%), liver cancer, biliary cancer (positive rate of 30%). Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: fasting Tips: When you draw blood, you should relax your mind and avoid the contraction of blood vessels caused by fear. Normal value <18.6 mg/L (radioimmunoassay). Clinical significance Increased in pancreatic cancer (positive rate 48% to 75%), acute pancreatitis (positive rate of 80%), chronic pancreatitis (positive rate of 15%), liver cancer, biliary cancer (positive rate of 30%). High results may be diseases: acute pancreatitis, pancreatic cancer, chronic pancreatitis, liver cancer precautions First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. 3. Please inform the doctor about the recent medication and special physiological changes before the test. Fourth, the separation of serum for examination, the specimen should not be hemolysis. Inspection process Immediately after blood collection, the inspection is carried out, and the inspection operation is strictly in accordance with the specifications. The basic procedure is to first wash the microplate with the anti-shhinocerebral antigen antibody, and then add the test specimen, the negative and positive reference serum, and after the heat preservation and washing, add the enzyme labeled anti-shhin embryo antigen antibody, and wash the same method. , add substrate color. For quantitative measurement, 100 μl of a substrate solution prepared by adding o-phenylenediamine and hydrogen peroxide per well was placed in a water bath at 37 ° C for 15 min to terminate the reaction, and the results were observed. For qualitative determination, it is best to use a substrate solution prepared with tetramethylbenzidine (TMB) and hydrogen peroxide, 100 μl per well, and placed in a 37 ° C water bath for 5 to 15 minutes. As a result, the A492nm value was read with an enzyme standard colorimeter. Quantitative determination can refer to the concentration of pancreatic embryo antigen in the standard solution as the abscissa, to determine the value of the hole A as the ordinate, draw a standard curve on the semi-logarithmic coordinate paper, and find the pancreas from the table according to the A value of the sample to be examined. Antigen content. Not suitable for the crowd Those without examination indications should not be tested. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.

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