Neisseria gonorrhoeae immunological detection

The gonococcal immunological assay is fluorescently labeled with a monoclonal antibody to the outer membrane protein PorI, the major antigenic site of Neisseria gonorrhoeae. The isolated strain was smeared for direct fluorescent staining, and apple green diplococcus was observed under a fluorescence microscope. Agglutinating bacteria can also be identified by agglutination or immunofiltration techniques using outer membrane protein monoclonal antibodies. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Tips: Avoid spicy food and strenuous exercise. Normal value Gram staining was positive in 53 cases, the detection rate was 39.26%, gonococcal culture was 68 cases, the detection rate was 50.37%, and PCR was positive in 78 cases, the detection rate was 57.78%. The above results were statistically processed x2 = 9.39, P0.05. Clinical significance Abnormal results: Neisseria gonorrhoeae is a Gram-negative diplococcus, oval or kidney-shaped, arranged in pairs, with a diameter of about 0.6-0.8 μm, often located in the cytoplasm of polymorphonuclear leukocytes (PMN), and in the chronic phase. Outside the cell. People who need to be examined: urinary tract sensation or burning sensation, redness and swelling of the urethra, and urinary tract irritation, such as frequent urination, urgency, dysuria, and thin or viscous secretions from the urethra. Positive results may be diseases: gonococcal conjunctivitis, gonococcal infection, gonorrhea, arthritis, gonococcal conjunctivitis Taboo before the test: eat spicy food and strenuous exercise. Requirements for inspection: follow the doctor's advice, use the medicine on time and in sufficient quantities, and choose the right medicine for the drug selection. For example, Yan Linqing is a regular treatment medicine. Inspection process 11% Triton-100 was stirred at room temperature (18-25 ° C) for 2 h, then ultrasonically pulverized (30 s × 8 times), centrifuged at 3000 r / min for 10 min, and the supernatant was collected. 2 ultrasonic pulverization: ultrasonic pulverization (30s × 8 times) in an ice water bath, centrifugation at 3000r / min for 10min, the supernatant was collected. 3 Freeze-thaw: Transfer the broth kept at -30 °C to room temperature, and then transfer to a -30 °C refrigerator after melting, repeat freezing and thawing 8 times in the same method, centrifuge at 3000 r/min for 10 min, and collect the supernatant. 4 Control: The bacterial solution was stored at 4 ° C without the above treatment, centrifuged at 3000 r / min for 10 min, and the supernatant was collected. Not suitable for the crowd People with sudden symptoms. Adverse reactions and risks Generally no adverse reactions.

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