Pepsin

The gastric juice contains PP and gastric protease, and the activity ratio of the two is 4:1, which is secreted by the main cells of the stomach, and is converted from pepsinogen (PG). PG is divided into two groups according to its electrophoretic mobility, called PGI and PGII, which can be released into the blood, and only PGI is discharged from the urine. PG is active in acidic gastric juices and inactivates at pH greater than 5. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Avoid eating a lot of food before checking. Normal value Metti method, PP40 ~ 60u. PGI 60.6 ± 13.9 mg / ml; PGII 11.9 ± 4.2 mg / ml, PGI / PGII ratio of 5.52 ± 1.86. Clinical significance Abnormal results: 1, PP: PP is mostly normal in gastric ulcer; duodenal ulcer is significantly increased; chronic duodenitis, gastric dilatation, chronic gastritis activity is weak; malignant anemia is extremely low or inactive. 2, PGI, PGII: increased levels suggest increased risk of peptic ulcer disease, increased PGI content is meaningful for judging ulcer activity. 3, PGI / PGII: The ratio was significantly lower in gastric cancer and atrophic gastritis, suggesting that this examination can be used as a trace indicator of atrophic gastritis and a screening method for early detection of gastric cancer. Low results may be diseases: chronic gastritis, reflux laryngitis, spleen, elderly gastric cancer, stress ulcer, trichinosis, high disease possible disease: chronic gastritis precautions Taboo before inspection: Eat a lot of food, affecting the inspection process. Requirements for examination: Pepsin isoenzyme levels in the blood can more accurately reflect the gastric mucosa. The patient is instructed by the doctor to do the relevant checks. Inspection process Operation method: Take 6 tubes, 3 of which are precisely added to the reference solution 1ml, and the other 3 are precisely added to the test solution 1ml, placed in a 37 ° C ± 0.5 ° C water bath, kept for 5 minutes, precision preheating to 37 ° C ± 0.5 The hemoglobin test solution at °C was 5 ml, shaken, and accurately timed, and reacted in a 37 ° C ± 0.5 ° C water bath for 10 minutes. Immediately add 5 ml of 5% trichloroacetic acid solution, shake well, filter, and take the filtrate for later use. Take another tube, add 5ml of hemoglobin test solution, place it in a 37°C±0.5°C water bath for 10 minutes, then add 5ml of 5% trichloroacetic acid solution, one of which is added with 1ml of test solution, and the other one Add 1 ml of the above hydrochloric acid solution, shake well, filter, and take the filtrate as the blank of the test sample and the control, respectively, and measure the absorbance at a wavelength of 275 nm by spectrophotometry (Appendix IVA) to calculate the average value AS and A, calculated by the following formula: A × Ws × n. Per 1g of protease-containing activity (unit) = As × W × 10 × 181.19, AS is the average absorbance of the control; A is the average absorbance of the test article; Ws is the tyrosine per 1 ml of the reference solution The amount, μg; W is the sample volume of the test sample, g; n is the dilution factor of the test sample. Under the above conditions, the amount of enzyme which hydrolyzes hemoglobin to produce 1 μmol of tyrosine per minute is a protease activity unit. Not suitable for the crowd Gastric perforation is prohibited in patients with gastric perforation. Adverse reactions and risks Generally no complications and harm.

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