Anti-soluble liver antigen antibody

Anti-soluble liver antigen antibody is a serological marker of type III autoimmune hepatitis, and has important value for the diagnosis and differential diagnosis of autoimmune hepatitis. Generally measured by indirect immunofluorescence. Principle of indirect immunofluorescence method: Label fluorescein on the corresponding antibody and directly react with the corresponding antigen. In the first step, add an unknown unlabeled antibody (sample to be tested) to a known antigen sample, and incubate at 37 ° C for 30 minutes in a wet box to fully bind the antigen and antibody, and then wash to remove the unbound antibody. In the second step, add fluorescently labeled antiglobulin antibodies or anti-IgG and IgM antibodies. If an antigen-antibody reaction occurs in the first step, the labeled antiglobulin antibody will further bind to the antigen-bound antibody, which can identify unknown antibodies.

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