Pepsin assay

The precursor of pepsin is pepsinogen, which is secreted by the main cells of the fundus gland and converted to gastric protease in gastric acid. Determination of pepsin can help diagnose stomach diseases. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in gastric cancer, atrophic gastritis, chronic gastritis. Gastric dilatation, chronic duodenitis, pernicious anemia, and hypothyroidism. Normal value: Serum pepsin: 40-60U/ml Above normal: No relevant information. negative: Positive: Tips: Fasting a lot of food before checking. Normal value 40 ~ 60U / ml. Clinical significance The decrease in pepsin secretion is seen in gastric cancer, atrophic gastritis, and chronic gastritis. Gastric dilatation, chronic duodenitis, pernicious anemia, and hypothyroidism. Low results may be diseases: chronic gastritis precautions Taboo before inspection: Eat a lot of food, affecting the inspection process. Requirements for examination: Pepsin isoenzyme levels in the blood can more accurately reflect the gastric mucosa. The patient is instructed by the doctor to do the relevant checks. Inspection process Take 6 tubes, 3 of which are precisely added to the reference solution 1ml, and the other 3 are precisely added to the test solution 1ml, placed in a 37 ° C ± 0.5 ° C water bath, kept for 5 minutes, precision preheating to 37 ° C ± 0.5 The hemoglobin test solution at °C was 5 ml, shaken, and accurately timed, and reacted in a 37 ° C ± 0.5 ° C water bath for 10 minutes. Immediately add 5 ml of 5% trichloroacetic acid solution, shake well, filter, and take the filtrate for later use. Another 2 test tubes were taken, and 5 ml of hemoglobin test solution was precisely added, and the mixture was moisturized for 10 minutes in a 37 ° C ± 0.5 ° C water bath. Then add 5 ml of 5% trichloroacetic acid solution, one of which is added with 1 ml of the test solution, and the other one is added with 1 ml of the above hydrochloric acid solution, shaken, filtered, and the filtrate is taken as the blank for the test and the control. In contrast, the absorbance was measured by spectrophotometry (Appendix IVA) at a wavelength of 275 nm, and the average values ​​AS and A were calculated and calculated by the following formula. A × Ws × n per 1g of protease-containing activity (unit) = As × W × 10 × 181.19 where AS is the average absorbance of the control; A is the average absorbance of the test article; Ws is the reference solution per 1 ml The amount of tyrosine, μg; W is the sample volume of the test sample, g; n is the dilution factor of the test sample. Under the above conditions, the amount of enzyme which hydrolyzes hemoglobin to produce 1 μmol of tyrosine per minute is a protease activity unit. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally not.

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