Complement C3 assay

Except for one subtype of C1 in the complement system, the other 10 are in an inactive state in the serum, and are activated only after activation. C3 is mainly produced in the liver, so its content changes reflect the function of the liver. C3 is reduced in patients with severe hepatitis and chronic active hepatitis. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Tips: If there is local congestion, use a warm towel after 24 hours to promote absorption. Normal value 1.14 ± 0.27 g / L. Clinical significance 1C3 is mainly produced in the liver, so its content changes reflect the function of the liver. C3 is reduced in patients with severe hepatitis and chronic active hepatitis. 2 C3 decreased in patients with acute glomerulonephritis, membrane proliferative glomerulonephritis and active systemic lupus erythematosus. 3C3 deficiency is seen in congenital testicular hypoplasia. A decrease in 4C3 content often indicates the occurrence of rejection. Precautions First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. If you have a history of fainting blood, please explain in advance and make special arrangements. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of dizziness, such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. Inspection process The amount of the diluent is calculated first according to the required dilution, and half of the amount is buffer. The anti-C3 serum was diluted with buffer and pre-warmed in a 56 ° C water bath. An equal amount of pre-warmed 15.0 g/L agar was then added to bring the final dilution of the antiserum to the desired level. The agar plates containing anti-C3 serum were routinely cast by a one-way immunodiffusion method, and the cells were perforated (pore size 3 mm) after cooling and solidification, and the pore spacing was 1.5 cm or more. The lyophilized C3 reference serum was added to an accurate amount of distilled water as specified, thoroughly mixed, and then diluted to 500 μg/ml with 0.1 g/L of thimerosal chloride physiological saline, followed by dilution. After making plates with anti-C3 serum at four dilutions of 1:25, 1:50, 1:75, 1:100, the above-mentioned different concentrations of C3 reference serum (10 μl per well) were added to the wells with a micro syringe. After incubation at 37 ° C for 48 h, the diameter of the precipitation ring was measured. Taking the diameter of the precipitation ring as the ordinate, the logarithm of the corresponding C3 content is the abscissa, and the standard curve is initially drawn. A standard curve drawn with a diameter of the precipitated ring of 4 to 8 mm is preferred. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.

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