DNA staining

Deoxyribonucleic acid is a component that makes up the nucleus. It is dyed in light red or purplish red with a special dye solution. Then its normal value should be: the accumulation, aggregation, deep staining of the DNA particles of the immature blood cells, the distribution of the deoxyribonucleic acid particles of the mature blood cells are uniform, small, and lightly stained. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: The DNA particles of naive blood cells are accumulated, aggregated, stained deeply, and the DNA particles of mature blood cells are evenly distributed, small and lightly stained. Positive: The abnormal result is positive, suggesting that there may be a blood disease such as leukemia. Tips: Immersion in DAB solution should be protected from light. Normal value The DNA particles of naive blood cells are accumulated, aggregated and stained deeply, and the deoxyribonucleic acid particles of mature blood cells are evenly distributed, fine and lightly stained. Clinical significance 1. Identify the maturity of the cells, such as the difference between small granulocytes and mature lymphocytes, small granulocytes stained lightly, nucleoli are obvious; lymphocytes stained deeply. 2, identify the type of acute leukemia, the original lymphocyte reaction is strong, the chromatin is dyed into coarse particles; the granulocyte nuclear reaction is weakened, the chromatin is lighter and finer granular; the original monocyte reaction is the weakest, the chromatin is slender. Shape, lighter staining. 3. Identification of megaloblasts and normal red blood cells. The nucleus of giant erythrocytes stained slightly finely, and the normal erythrocyte nucleus stained deep into coarse particles to massive. Positive results may be diseases: acute leukemia, acute lymphocytic leukemia considerations In operation, 4-6 should be washed twice with 0.1 mol/L phosphate buffer each time to avoid sediment. Immersion in the DAB solution should be protected from light. DAB is a carcinogen should pay attention to skin protection. Inspection process 1. Fresh anticoagulant specimens were separated by density gradient using a lymphocyte separation solution with a specific gravity of 1.077. The lymphocytes were centrifuged and lysed. Or do not separate the cells, directly smear. 2. The smear was fixed with hydrogen peroxide-methanol solution at 4 ° C for 20-30 min to block the endogenous peroxidase of the cells. 3. The smear was immersed in 0.1 mol/L phosphate buffer for 15 min, and normal rabbit serum was added dropwise to reduce non-specific binding of the antibody. 4. Rabbit anti-bovine TdT antibody was added dropwise and incubated at 37 ° C for 30 min. 5. Add goat anti-rabbit IgG antibody and incubate at 37 ° C for 30 min. 6. Add PAP immune complex and incubate at 37 °C for 30 min. 7. Dip the smear into the DAB solution and let it sit for 5-10 minutes at room temperature. 8. Wash for 5 minutes, dry microscopic examination, or counterstain the nucleus with Hastelloy hematoxylin solution for 10 min. Not suitable for the crowd There are no special taboos. Adverse reactions and risks This test generally does not cause complications or harm.

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