von Willebrand factor

A single antiserum of factor VIII-related antigen (VIIIR:Ag) was mixed with agar to prepare a thin plate, and a certain amount of plasma (antigen) was added. After electrification, a specific rocket-like immunoprecipitation peak was formed under electroacoustic action. The height of the peak is proportional to the antigen concentration. Strenuous exercise should be avoided before testing, and anticoagulant drugs such as aspirin should not be taken. Basic Information Specialist classification: examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Avoid vigorous exercise before testing. Do not take anticoagulant drugs such as aspirin. Normal value 79% to 117%. Clinical significance (1) VWF: Ag content increased in hypercoagulable state and thrombotic diseases, for example, acute myocardial infarction, angina pectoris, hypertension, pulmonary heart disease, cerebrovascular disease, diabetes, glomerulonephritis, nephrotic syndrome, cirrhosis, Uremia, pregnancy, pregnancy-induced hypertension syndrome, malignant tumor, DIC, etc. (2) VWF: Ag content reduces vascular hemophilia and the like. Low results may be diseases: high results of nephrotic syndrome may be diseases: von Willebrand disease, cerebrovascular disease, acute myocardial infarction, pulmonary heart disease, malignant tumor, angina pectoral precautions Strenuous exercise should be avoided before testing, and anticoagulant drugs such as aspirin should not be taken. Inspection process (1) Take a certain amount of VWF:Ag antiserum, dilute 10-fold with Tris-barbital buffer, and take 5g/L agarose Tris-barbital buffer for water. Melt and cool to 56 °C. Then, the diluted VWF:Ag antiserum pre-warmed to 56 ° C was mixed 1:60 (depending on the antibody titer) to prepare an agar plate of 8 cm × 8 cm and a thickness of 1.5 mm, and a row of about 2 cm from the side of the glass plate. Small hole, diameter hole 3mm, hole spacing is 5mm. (2) Place the agar plate in the electrophoresis tank, the hole toward the cathode, use the filter paper as a salt bridge, and use a quantitative syringe to absorb the healthy human diluted with different ratios of 1:2, 1:4, 1:8, 1:16, etc. 10 μl of each plasma was added to the wells of the antibody agar plate. The plasma samples to be tested were diluted 1 time with Tris-Barbital buffer and added to each sample well. The amount per well was 10 μl. (3) Electrophoresis conditions: electrophoresis 110 ~ 150V, current about 5mA / plate, time 18h, temperature below 15 °C. (4) After electrophoresis is completed, the gel plate is taken out and immersed in 4.4mmol/L phosphomolybdic acid dyeing solution for more than 20min, and then soaked in physiological saline for 10min, the obvious rocket-like precipitation peak can be seen, and the amino black can also be used as usual. dyeing. (5) After staining, measure the height of the rocket electrophoresis peak with double gauge (from the upper edge of the sample well to the peak), and take 5 readings of standard plasma, and obtain a standard curve by regression equation, and then find each sample VWF. The content of Ag is multiplied by the dilution factor (×2), which is the actual concentration of the sample. Indicates the percentage equivalent to normal plasma. Not suitable for the crowd There is no inappropriate crowd. Adverse reactions and risks It is a safe check and is harmless to the body.

The material in this site is intended to be of general informational use and is not intended to constitute medical advice, probable diagnosis, or recommended treatments.