Anti-acetylcholine receptor antibody (AchR-Ab)

Anti-acetylcholine receptor antibodies are mainly IgC type (mainly IgCl, IGC.2 subclass, followed by IgC. subclass), and IgM type can be detected. Serum anti-AchR antibodies of different types of MG patients have different properties. The first method of anti-AchR antibody detection is radioimmunoassay. Later, in order to avoid the use of nuclides, various enzyme-linked immunosorbent assays have emerged. These detection methods must be applied. A-bungarotoxin (a-BT) a-BT is a peptide extracted from the venom of the silver snake, with a molecular weight of about 8kD. It is highly selective in combination with AchR and this combination is almost irreversible. For example, by ELISA, the solid phase vector can be pre-coated with ct-BT, and then the AchR added by specific adsorption can be used to measure the anti-AchR antibody in the serum to be tested. Basic Information Specialist classification: neurological examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Positive (>0.169): myasthenia gravis. In addition, the detection rate was highest in patients with myometrial weakness, systemic or thymoma-free systemic myasthenia gravis with thymoma, and the positive rate was over 70%. Tips: After 8 pm on the day before the medical examination, you should start fasting for 12 hours, so as not to affect the test results. Normal value The ELISA method was 0.09 to 0.148 or negative. (Note the specific reference value depends on each laboratory.) Clinical significance Abnormal results were positive (>0.169) myasthenia gravis. In addition, the detection rate was highest in patients with myometrial weakness, systemic or thymoma-free systemic myasthenia gravis with thymoma, and the positive rate was over 70%. Need to check the glandular disorders of the population, muscle contraction is weak. Positive results may be diseases: myasthenia gravis, acquired immunodeficiency syndrome considerations Before the test: Please inform the doctor about the recent medication and special physiological changes. 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Note: The positive rate of anti-acetylcholine receptor antibody is also related to the clinical type of myasthenia gravis, race and other autoimmune diseases. Inspection process Anti-AchR antibodies are detected by sensitive ELISA method: coated with α-bungarotoxin and labeled with skeletal muscle homogenate (including AchR), then added to the test serum and control serum, and finally added with enzyme label antibody. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.

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