Anti-pancreatic acinar antibody (PAB)

Anti-pancreatic acinar antibodies (PAB) have certain value in the diagnosis and differential diagnosis of ulcerative colitis and Crohn's disease. Anti-pancreatic acinar antibodies are commonly determined by indirect immunofluorescence in clinical practice. The principle of indirect immunofluorescence experiments is to label fluorescein on the corresponding antibody and directly react with the corresponding antigen. In the first step, an unknown unlabeled antibody (sample to be tested) is added to a known antigen sample, and incubated at 37 ° C for 30 min in a wet box to sufficiently bind the antigen antibody, followed by washing to remove unbound antibody. In the second step, a fluorescently labeled anti-globulin antibody or an anti-IgG, IgM antibody is added. If an antigen-antibody reaction occurs in the first step, the labeled anti-globulin antibody will further bind to the antigen-bound antibody, thereby identifying an unknown antibody. Basic Information Specialist classification: Digestive examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The hint may be Crohn's disease. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Normal value Indirect immunofluorescence was negative. (Note the specific reference value depends on each laboratory.) Clinical significance Abnormal results are positive: 1. High titer anti-pancreatic acinar antibodies are mainly found in Crohn's disease, the incidence rate is 39%, and the antibodies are mainly IgG and IgA. Anti-pancreatic acinar antibodies can be present in patients with pancreatitis, but the titer is low. 2. Patients with Crohn's disease who are anti-pancreatic acinar antibody-positive are more susceptible to pancreatic exocrine function damage than patients with negative antibody. 3. When this antibody is simultaneously measured with anti-neutrophil antibodies, the diagnostic accuracy of Crohn's disease can be increased from 39% to 43%. Patients with Crohn's disease and pancreatitis need to be examined. Positive results may be diseases: autoimmune pancreatitis, pancreatitis precautions Forbidden before examination: Please inform the doctor about the recent medication and special physiological changes before the test. 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Requirements for examination: When taking blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Inspection process The principle of indirect immunofluorescence experiments is to label fluorescein on the corresponding antibody and directly react with the corresponding antigen. In the first step, an unknown unlabeled antibody (sample to be tested) is added to a known antigen sample, and incubated at 37 ° C for 30 min in a wet box to sufficiently bind the antigen antibody, followed by washing to remove unbound antibody. In the second step, a fluorescently labeled anti-globulin antibody or an anti-IgG, IgM antibody is added. If an antigen-antibody reaction occurs in the first step, the labeled anti-globulin antibody will further bind to the antigen-bound antibody, thereby identifying an unknown antibody. Not suitable for the crowd Abnormal people: generally no special population. Adverse reactions and risks No related complications or hazards.

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