Anti-keratin antibody (AKA)

Anti-keratin antibody (AKA), Young et al found in 1979, found that rheumatoid arthritis (RA) serum has an antibody that reacts with the rat's esophagus, and is specific for rheumatoid arthritis (RA). For AKA. In 1989, Vincent et al. suggested that AKA should be renamed as an anti-keratinocyte antibody. AKA can occur several years before the onset of RA, so it has early diagnostic value. AKA method was used to take the lower third of the esophagus of 6-week-old male Wistar rats as an antigen, and to make frozen sections, which were 4 μm to 5 μm thick, and stored at -70 °C for use. The serum was diluted 1:20, incubated in a humid box at 37 ° C for 30 minutes, rinsed with PBS, blown dry, and fluorescein-labeled goat anti-human IgG diluted 1:20, incubated at 37 ° C for 30 minutes, rinsed, blow dried, buffered glycerin seal Tablets were observed under a fluorescence microscope. The criteria for the determination were positive for linear or lamellar fluorescence with typical regularity in the stratum corneum. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Positive systemic lupus erythematosus, rheumatoid arthritis, Sjogren's syndrome, systemic sclerosis, ankylosing spondylitis. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value IBT method (immunoblotting) and IIF method (indirect immunofluorescence) were negative. Clinical significance Positive systemic lupus erythematosus, rheumatoid arthritis, Sjogren's syndrome, systemic sclerosis, ankylosing spondylitis. Note that anti-keratin antibodies are mainly found in patients with rheumatoid arthritis, with a positive rate of 36 to 59% and a specificity of 95 to 99%. Because of the low sensitivity, negative results cannot rule out the diagnosis of rheumatoid arthritis. Anti-keratin antibodies are rare in other non-rheumatoid arthritis as well as non-inflammatory rheumatism. Anti-keratin antibodies can occur before rheumatoid arthritis (RA) is undiagnosed. The antibody-positive group was finally diagnosed with RA after half or more of follow-up, and the number of non-RA diagnosed was small. Conversely, the number of cases diagnosed as rheumatoid arthritis (RA) in the antibody-negative group was small, and the number of cases diagnosed as non-rheumatoid arthritis (non-RA) increased significantly. AKA antibodies can occur in patients with undetermined joint pain/arthritis and have a value in early diagnosis of RA. AKA needs to be combined with other rheumatoid autoantibodies. If the number of autoantibodies is more, the possibility of RA is greater. In particular, it has complementary diagnostic significance for patients with RF-negative RA. Positive results may be diseases: precautions for rheumatoid arthritis in the elderly First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. 3. Please inform the doctor about the recent medication and special physiological changes before the test. Inspection process Same as ELISA. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.

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