Factor B hemolytic activity

When the alternative pathway of complement activation (AP) is activated, the components of the pre-complement (C1, 4, 2) are not activated. In addition to C3 ~ C9 involved in AP activation, there are factors such as P, D, B. Basic Information Specialist classification: Oncology examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: REs of different individuals have an effect on the results of the assay. Normal value 83% to 121%. Clinical significance The complement alternative pathway is activated, and the involved components are complement C3, C5～C9, P factor, D factor, B factor, etc., and any abnormality of any component can cause the change of hemolytic activity of the alternative pathway. The hemolytic activity of AP-CH50 is significantly increased in some autoimmune diseases, nephrotic syndrome, chronic nephritis, tumors, infections, etc., and reduction is seen in cirrhosis, slow-lived liver, acute nephritis and the like. Precautions (1) Complement instability in serum is easy to destroy. It can only be placed for 1 day at 4 °C, 5 days at 0 °C, and can be placed for 1 month at -20 °C. (2) The excessively high concentration of Mg2+ in the diluent will affect the activity of AP-CH50. (3) pH of the diluent: The optimum pH is 7.2 to 7.5, otherwise the results will be affected. (4) RE of different individuals have an effect on the measurement results. Inspection process Determination of B-factor hemolytic activity: Unsensitized rabbit red blood cells (RE) (having similar zymosan or inulin to human complement) can activate AP in human serum diluted with Mg2+-containing EGTA buffer, causing hemolysis . The degree of hemolysis reflects AP activity. Reagents: (1) 0.1 mol/LEGTA: 3.5 g of NaOH was taken, dissolved in 85 ml of distilled water, and 19 g of EGTA (ethylene glycol diethylamine ether tetraacetic acid) was added to dissolve it, and distilled water was added to 500 ml. (2) Barbital buffer stock solution: NaCl 121.25 g, barbital 1.44 g, barbital sodium 0.94 g, distilled water to make up 500 ml. (3) Diluent: 0.1 mol/LEGTA 80 ml, 180 ml of barbital buffer solution, 0.41 g of MgCl 2 ·6H 2 O, distilled water to 1000 ml, and adjusted to pH 7.5 with 1 mol/L NaOH. (4) 0.5% RE suspension: The RE stored in the Alsever solution was washed twice with physiological saline, and the diluted solution was washed once to prepare a 0.5% suspension (RE3 × 108 / ml). (5) Tested serum: The serum to be tested is stored at -20 ° C immediately after separation, diluted with a dilution of 1:4 before use, and a water bath at 37 ° C for 10 min. (6) 50% hemolysis standard tube: 0.5% RE 0.2 ml, and 0.8 ml of distilled water was added. Method of operation: Each reaction solution was added according to the table. Not suitable for the crowd There are no taboos. Adverse reactions and risks No related symptoms and hazards.

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