Hepatitis B e Antigen

Hepatitis B virus e antigen (HBeAg) is a component of HBV core antigen, and HBeAg is an indicator of hepatitis B virus replication, which indicates that hepatitis B virus replication is active and viral load is high. When HBeAg turns negative or is accompanied by anti-HBe conversion, it is often accompanied by a decrease in viral load and an improvement in liver function. However, some patients still maintain active replication of the virus after HBeAg is negative, and the viral load and liver inflammation activity are still high. These patients are called HBeAg-negative chronic hepatitis B. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Positive is abnormal, HBeAg positive indicates active replication in hepatocytes, and serum is highly contagious. HBeAg is transiently positive in acute hepatitis B, generally no more than 10 weeks, such as continuous positive suggesting that it becomes chronic. Tips: You must take a blood test on an empty stomach before the test. Do not eat for 8-12 hours before blood draw, but drink a small amount of water. It is best to ban alcohol and drugs the day before the blood test. Normal value Normally negative. The Cutoff value was calculated as COV = negative control OD value x 2.1. OD ≥ COV is positive, the specimen OD value. Note (The negative control OD value is lower than O.05 for 0.05 calculation, and higher than 0.05 for actual OD value). Clinical significance HBeAg positivity indicates active replication in hepatocytes and serum is highly contagious. HBeAg is transiently positive in acute hepatitis B, generally no more than 10 weeks, such as continuous positive suggesting that it becomes chronic. Positive result may be disease: hepatitis B precautions (1) HBeAg positive is a sign of hepatitis B infection. Detection of HBeAg helps to determine the infectious power of HBsAg carriers, the risk of mother-to-child transmission and the prognosis of acute hepatitis B. The disappearance of HBeAg and the appearance of anti-HBe show that hepatitis B is improving, but it cannot be used as an indicator of non-infectiousness, and it is not necessarily a sign of beneficial to the body (alpha-fetoprotein is related to anti-HBe). (2) The result of the competition method for detecting anti-HBe is judged by the colorimetric method. The visual method cannot correctly judge the critical value of the sample. Inspection process Follow the kit instruction manual. (1) Two-step operation: 1 Adding sample: When detecting HBeAg, 100 μl of the sample was added to each well of the coated reaction plate (strip), and each test had a negative and positive control, and the plate was incubated at 37 ° C for 2 h, and the plate was washed 4 times. When anti-HBe was detected, 50 μl of the neutralizing reagent and 50 μl of the sample were added to each well. Each test had an yin and a positive control, and the plate was incubated at 37 ° C for 2 h, and the plate was washed 4 times. 2 Anti-HBe-HRP was added: 100 μl of anti-HBe enzyme conjugate was added to each well, and the plate was incubated at 37 ° C for 2 h, and the plate was washed 4 times. 3 Color development: 100 μl of the substrate solution was added to each well, and the mixture was incubated at 37 ° C for 15 min, and then a stop solution of 1 mol/L sulfuric acid 50 μl was added. 4 Results interpretation: A. Visual method: When detecting HBeAg, the colorlessness is negative, and the pale yellow to orange color is positive. When HBe was detected, the orange color was negative, and the colorless and pale yellow were positive. B. Colorimetric method: On the microplate reader, the zero point of the reagent blank is used to measure the optical density value of each hole at 492 nm. When detecting HBeAg: When it is greater than or equal to 2.1, it is positive, otherwise it is negative. When detecting anti-HBe: When it is greater than or equal to 2.1, it is positive, otherwise it is negative. (2) One-step operation: 1 plus sample: add neutralizing reagent and anti-HBe-HRP. A. Detection of HBeAg: 50 μl of the sample and 100 μl of anti-HBe-HRP were added to each well of the coated reaction plate (strip), mixed and incubated at 37 ° C for 1 h, and the plate was washed 4 times. B. Detection of anti-HBe: 50 μl of the sample was added to each well, 50 μl of the neutralizing reagent and anti-HBe-HRP were mixed, and the mixture was incubated at 37 ° C for 1 h, and the plate was washed 4 times. 2 color development and interpretation results: the same two-step method. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally no complications and harm.

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