Hepatitis B e antibody

Hepatitis B virus e antibody (HBeAb) is an antibody corresponding to HBeAg in the body, and its appearance proves that the body has certain immune scavenging ability to HBeAg. Hepatitis B e antibody was positive for several months after the hepatitis B e antigen was negative. In general, hepatitis B e antibody positive indicates that the patient's infectivity has been significantly or relatively reduced. The degree of viral replication has decreased or has been significantly alleviated. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: The Cutoff value was calculated as COV = negative control OD value x 0.3, and sample OD value ≥ COV was negative. Positive: The Cutoff value calculates the COV = negative control OD value x 0.3, and the sample OD value is less than COV positive. Tips: You must take a blood test on an empty stomach. Do not eat for 8-12 hours before blood draw, but drink a small amount of water, especially for those who check liver function for the first time. Normal value The Cutoff value was calculated as COV = negative control OD value x 0.3. The sample OD value ≥ COV is negative, and the sample OD value is less than COV is positive. Clinical significance When HBeAg is negative, HBeAb is often indicated that HBV replication is stopped or significantly weakened, and the infection is weak. It is a sign that acute hepatitis B is improved and the prognosis is good. However, the pre-C region gene mutation can produce HBV infection with HBeAg negative conversion, HBeAb can be positive, the virus is still active, and the lesion can progress continuously. Positive results may be diseases: chronic hepatitis, hepatitis B precautions Before inspection: 1) Blood must be taken on an empty stomach. Do not eat for 8-12 hours before blood draw, but drink a small amount of water, especially for those who check liver function for the first time. 2) It is best to ban alcohol and drugs the day before the blood test. Inspection process 1, according to the material: blood. 2, the principle of determination of hepatitis B e antibody The monoclonal anti-HBe coated reaction plate was added to the specimen to be inspected, and the genetically engineered recombinant HBeAg and the polyclonal anti-HBe-HRP were added to form a competitive binding. If the anti-HBe content in the sample to be tested was high, the anti-Hbe was obtained. -HRP is combined with HbeAg to form a free form which is washed away. When TMB substrate is added, the color is light, and the color is dark. 3, reagents Coated anti-HBs reaction plate and HRP-anti-HBs, negative, positive control serum, washing solution, substrate solution, and the like. 4, the operation method (l) Experimental preparation The kit was taken out from the refrigerated environment, equilibrated at room temperature for 30 min, and the concentrated washing solution was diluted 1:20. (2) Add the sample to be tested to 0.05 ml per well of the specimen to be tested, and set 2 wells for the positive control and 1 well for the blank control for the negative control. (3) Add 0.05 ml per well to the neutralizing reagent, and the blank control well is not added. (4) Add 0.05 ml of the enzyme conjugate per well, blank control wells are not added, mix well, incubate at 37 ° C for 30 min. (5) Washing machine wash plate Select washing process 5 times to wash the plate, and finally pat dry. (6) Add coloring agent first to add coloring agent A, 0.05ml per well; add coloring agent B, every 0.05ml; mix thoroughly, place at 37 °C and incubate for 15 minutes in the dark. (7) Stop the reaction Add 0.05 ml of stop solution to each well and mix. (8) For the determination of the microplate reader, the single-wavelength 450 nm (with blank hole calibration) or dual wavelength 450/630 can be selected to read the OD value of each well. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally no complications and harm.

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