Hepatitis B Surface Antibody

Hepatitis B surface antibody (HBsAb) is produced by both HBV infection and immunologically injected HBV vaccine. HBsAb has two types, IgM and IgG. The former occurs in the early stage of infection, and the latter occurs later. It is a protective antibody with good neutralization and long-term presence in serum. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: The human body has no immune effect on hepatitis B virus. Positive: Positive indicates that the body has certain immunity to hepatitis B virus. Tips: You must take a blood test on an empty stomach. Do not eat for 8-12 hours before blood draw, but drink a small amount of water. Normal value negative. Clinical significance Anti-HBs are the main protective antibodies after HBV infection, which usually appear after the disappearance of HBsAg, and slowly rise during the recovery period of hepatitis B, which can last for many years. Anti-HBs positive indicates that acute hepatitis B or latent infection has recovered and has immunity to HBV. After injection of hepatitis B vaccine, anti-HBs in serum is a sign of immune success. Positive result may be disease: hepatitis B precautions (1) Shake the reagent before use, and discard l~2 drops and then add it vertically. Pay attention to even force. (2) The kit taken out from the refrigerated environment should be equilibrated at room temperature for 30 minutes before testing. The rest should be sealed in time and stored in the refrigerator for later use. (3) The specimen to be inspected for refrigeration should be equilibrated at room temperature for 30 minutes and then tested. (4) NaN3 antiseptic is not available for the specimen to be inspected. Inspection process (1) Use a carbonate buffer for proper dilution of anti-HBs·IgG (with anti-HBs serum or with a kit) to coat the polystyrene reaction plate, add 0.1ml per well (the last hole is not added) , as a blank). Cover and put at 4 ° C for 24 h. The next day, the cells were washed three times with Tris-HCl-Tween 20 buffer, and finally the plate was placed on a blotting paper to allow the liquid to flow out. (2) Add 0.1 ml of the serum to be tested to each well (observe the titer for different dilution). Positive and negative serum controls were performed simultaneously on each plate. After capping, the solution was removed at 37 ° C for 2 h, and washed three times as above. (3) Add the substrate solution (0.1mol/L Na2HPO45.14ml, 0.05mol/L citric acid 4.86ml, o-phenylenediamine 4mg, 3% H2O20.05ml, protected from light, ready for use) 0.1ml. Dark in room temperature for 15 to 30 minutes. After the positive control wells were fully developed, the reaction was stopped by adding 2 mol/L H2SO4 0.05 ml to each well. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally no complications and harm.

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