Virus Infection Immunoassay

Viral infection is common in clinical practice, but due to the strict intracellular parasitism of the virus, the course of disease is rapidly proliferated and changes rapidly. The isolation and identification of complex viruses and traditional serological examinations are difficult to be used as routine laboratory tests and cannot meet clinical diagnosis. demand. The rapid diagnosis and molecular biology techniques that have emerged in recent years have opened up new methods for clinical virological examination and provided convenience. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The result is abnormal and the chance of illness is large. Tips: banned overeating and strenuous exercise. Normal value The result was negative after the examination. Clinical significance Abnormal results: After the virus infects the cells, it can cause different cell changes, and the unique cytopathic effect caused by the proliferation of the virus in the cells is called cytopathic effect (CPE). Common CPE has cell shrinkage, aggregation, drawing, necrosis and shedding, which is the most important indicator for virus proliferation. Secondly, cells can be fused into multinucleated giant cells, such as cytomegalovirus. Some viruses (such as rabies virus) grow and proliferate in cells, and can form inclusion bodies in the cytoplasm or nucleus. People who need to be examined: those infected with the virus and the immune system is destroyed. Positive results may be diseases: congenital rubella syndrome, congenital rubella, epidemic hemorrhagic fever, pregnancy with cytomegalovirus infection, pharyngeal membrane fever, indigestion, milk hemp, big head lice, bird flu, human avian influenza precautions Taboo before inspection: prohibiting overeating and strenuous exercise, should be fasting, in a calm state. Requirements for examination: It should be tested within 1~2 days of onset. The virus is easy to lose activity at room temperature. It should be stored at low temperature after collection and sent for inspection as soon as possible. It is best to send it within 1~2 hours after collection. Inspection process In recent years, immunolabeling techniques, such as fluorescein, radionuclide, and peroxidase, have been used to detect antigens in specimens for early diagnosis of viral infection. The method has the advantages of sensitivity, specificity and speed. Because immunofluorescence technology requires fluorescence microscopy, and sometimes false positives; solid phase radioimmunoassay can cause radioactive contamination and must use special radioactive laboratory and other shortcomings, so the clinical use of enzyme-linked immunosorbent assay (ELISA) is important. The diagnosis of significance needs to be confirmed by Western blotting (westernblor). Not suitable for the crowd Inappropriate people: no special requirements. Adverse reactions and risks No related complications or hazards.

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