Quantitative Transparent Method for Parasite Examination

The quantitative transparency method for parasite inspection is an auxiliary examination method for detecting parasites. The principle is to use fecal quantitative or qualitative thick smear to increase the number of eggs in the field of vision, and can be used for quantitative examination of eggs. Treated with glycerin and malachite green, the fecal membrane is transparent, so that the feces and the eggs are in sharp contrast, which is convenient for light transmission and microscopic examination. Malachite Green softens the field of view to reduce eye strain. Suitable for checking various worm eggs. The method is simple and the eggs will not be lost during the operation. The effect is better. This method should not be used for hard and too thin feces. Foamy feces can form many tiny bubbles under the cellophane, which prevents microscopic examination. It can also be used for quantitative inspection of eggs. The quantitative transparent method is applicable to the inspection and counting of various worm eggs in the feces, and can measure the degree of infection of worms in the human body (Wormburden), and can also judge the effect of drug deworming. This phase check can be used to determine the corresponding symptoms. Basic Information Specialist classification: Digestive examination classification: feces / parasitic examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative results suggest that it is generally normal. Positive: A positive test result indicates a parasitic infection. Tips: Do not take anti-parasitic drugs before inspection, so as not to affect the results. Normal value No parasite eggs were found to be normal during the examination. Clinical significance Abnormal results detected parasite eggs. People who need to be examined have abnormal gastrointestinal tracts. Positive results may be diseases: biliary ascariasis, appendicial parasitic diseases Contraindications before the test: Do not take anti-parasitic drugs before the test, so as not to affect the results. Inspection requirements: Fecal examination is a common pathogen detection method for the diagnosis of parasitic diseases. To get accurate results, the stool must be fresh and the inspection time should not exceed 24 hours. If you check the protozoa trophozoites in the intestines, it is best to check them immediately, or temporarily store them at 35 °C ~ 37 °C. Containers containing feces must be clean, dry, and prevent contamination; feces should not be mixed with urine and other body fluids, so as not to affect the results of the inspection. Inspection process This method uses modified polystyrene as a quantitative plate, the size is 40×30×1.37mm, the die hole is a long round hole, the size is 8×4mm, the ends are semi-circular, and the fecal sample taken is 41.7mg on average. . During operation, a 100 mesh nylon mesh or metal mesh having a size of about 4×4 cm is covered on the stool specimen, and the stool is scraped off from the sieve mesh, and the quantitative plate and the slide glass are placed on the sieve plate, and the quantitative plate is pressed by the two fingers of one hand. At both ends, the feces on the scraper are filled with the mold holes, and the excess feces are scraped off. Pick up the dosing plate, leave a long fecal sample on the slide, and then cover the dung strip with a glycerin-malachite green solution of about 5.0-2.6 cm in size. After flattening and pressing, make the cellophane under the cellophane. The stool is grown in an oval shape. After 1 to 2 hours, the feces were transparent and counted under the microscope. The number of eggs obtained is × 24, and the above coefficient of fecal traits is the number of ovulations per female per gram of common worms per gram of egg worm (EPG). Not suitable for the crowd The test is non-invasive and generally has no contraindications. Adverse reactions and risks The test is non-invasive and will not have serious complications or other hazards.

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