Schistosoma spot ELISA

In the immunological diagnosis of schistosomiasis, almost all kinds of immunological tests have been tried. In addition, there are cercaria membrane test and ring egg sedimentation test using Schistosoma cercariae and eggs as antigens. An enzyme-linked immunosorbent assay with high sensitivity, specificity and wide use is now introduced. Basic Information Specialist classification: Infectious disease inspection and classification: feces / parasitic examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The positive rate is 89% to 97%; there is a certain cross-reaction to the serum of patients with paragonimiasis and liver fluke. Tips: DOT-ELISA is highly sensitive and can be tested on a wide range. It has been tested for the diagnosis of various parasites. Normal value Negative colorless. Clinical significance Can be used as an auxiliary diagnosis of schistosomiasis patients. The positive rate is 89% to 97%; there is a certain cross-reaction to the serum of patients with paragonimiasis and liver fluke. Positive results may be diseases: visceral schistosomiasis, schistosomiasis, vulvar schistosomiasis, schistosomiasis precautions DOT-ELISA is highly sensitive and can be tested on a wide range and has been tested for the diagnosis of various parasites. Inspection process In the same indirect ELISA, in principle, a 1% dilution of 1% Schistosoma japonicum soluble egg antigen was coated with polystyrene plates, 200 μl per well, placed at 4 ° C overnight, washed, and then added with Tween-PBS for 1: 200 diluted serum samples, 200 μl per well of negative and positive reference serum. Each specimen should be at least duplicated. The control group was not added with serum, only PBS Tween was added, and after incubation, the appropriately diluted enzyme-labeled anti-human IgG was added, 200 μl per well. After the heat preservation and washing, the color of the A492 was read by an enzyme-based colorimeter after coloring according to the conventional substrate. The zero point was corrected by a mixture of 4 parts of o-phenylenediamine (OPD) substrate and 1 part of 2 mol/L of sulfuric acid (corrected A value greater than or equal to 0.5 is a positive reaction. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No related complications or hazards.

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