Hemoglobin solubility

The anti-alkaline and anti-acidic properties of HbF are stronger than normal hemoglobin (HbA). Therefore, after the blood is eluted by acid, the red blood cells containing HbF are not discolored due to acid resistance, and can be dyed red by eosin. Red blood cells containing HbA or only a small amount of HbF are eluted by acid, leaving only the empty shadow of the erythrocyte membrane. The blood samples of severe beta-thalassemia are similar to those of neonates. Light beta-thalassemia has only a few eosin-positive red blood cells, and the staining is different. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value 88% to 102% (average 94.4%). Clinical significance Hemoglobin disease heterozygous (A/S) is 35% to 68% (average 51.6%). Hemoglobin disease heterozygous (S/F) 6% to 23% (average 14.6%). Hemoglobin disease heterozygous (S/C) is 36% to 44% (average 40.4%). Hemoglobin disease heterozygous (A/C) is 83% to 103% (average 93%). Precautions (1) The solubility of different abnormal hemoglobin is different, and the solubility of several heterozygous abnormal hemoglobin is HbA>HbG>HbS. Therefore, the reduction of pure solubility is not enough to diagnose HbS, and should be combined with other examinations. (2) False positive results can be seen in hyperlipidemia. Multiple myeloma, cryoglobulinemia. False negative results can be seen in the expiration of the reagent. A large number of recent blood transfusions, newborns and infants under 6 months. (3) HbCHarlem, HbTrais and HbCZiguinchor may also have positive results. Inspection process (1) Na2S2O420mg was placed in a test tube, and 1.8 ml of a 2.5 mol/L phosphate buffer solution was added. After mixing and dissolving, 0.2 ml of the hemoglobin solution to be tested was added. Mix again and let it filter at room temperature for 15 min or more. 0.2 ml of the filtrate was added and added to 3.8 ml of a 2.5 mol/L phosphate buffer solution in which 420 mg of Na2S2O was dissolved. This tube is the tube to be tested. (2) Take 0.02 ml of dissolved blood and add 4 ml of distilled water, which is a control tube. (3) Zeroing with distilled water, and measuring the absorbance of the measuring tube and the control tube at a wavelength of 420 nm. Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.

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