Hemoglobin F inclusion body formation assay

The redox dye tar tar blue was added to the blood, and after incubation at 37 ° C, HbH precipitated due to oxidative degeneration, was granular, was dyed dark blue, and was uniformly and diffusely dispersed in the red blood cells. In patients with HbH disease, red blood cells containing inclusion bodies can reach more than 50%. In the case of mild thalassemia, HBH inclusion bodies can occasionally be seen. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: This test is affected by temperature and should be paid special attention. Normal value After 1 hour of light heat preservation, the red blood cells containing HbF inclusion bodies were <5%. Clinical significance (1) >50% HbF hyperplasia homozygous. (2) > 5% unstable hemoglobin disease, alpha-thalassemia light type. Precautions This test is affected by temperature and should be given special attention. Inspection process Counting method. Not suitable for the crowd Hemophilia and diffuse intravascular coagulation. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.

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