Fibrinogen assay

Fibrin, coagulation factor I, is primarily synthesized by the liver and is involved in the coagulation process. When fibrinogen exceeds the normal range, it indicates abnormal blood coagulation. Acquired pregnancy poisoning, acute infection, shock, malignant tumor, multiple myeloma, cerebral infarction, acute myocardial infarction, atherosclerosis, deep vein thrombosis, hyperlipidemia, radiation therapy or postoperative surgery, burn, collagen Sexual diseases, diabetes with vascular disease, glomerular disease active period, nephrotic syndrome, rheumatic fever, tuberculosis, fibrinogen-containing blood preparations, such as AHG anti-human globulin preparation, fresh frozen plasma, etc. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Blood collection should not be >45s, and the first 2ml should be discarded. The syringe should be silicided or plastic syringe. Normal value 2 to 4 g/L (0.2 to 0.4 g/dl). Clinical significance (1) Increase in hypercoagulable state 1 Physiological elderly, women's menstrual period, late pregnancy, oral administration of estrogen, exercise, etc. 2 acquired gestational toxicosis, acute infection, shock, malignant tumor, multiple myeloma, cerebral infarction, acute myocardial infarction, atherosclerosis, deep vein thrombosis, hyperlipidemia, radiation therapy or post-operative, burn, Collagen diseases, diabetic vasculopathy, glomerular disease active period, nephrotic syndrome, rheumatic fever, tuberculosis, fibrinogen-containing blood preparations, such as AHG anti-human globulin preparation, fresh frozen plasma, etc., are input in a long period of time. (2) reduction 1 physiological newborn. 2 congenital A. no fibrinogenemia. B. Low fibrinogenemia. C. Part of abnormal fibrinogenemia. 3 acquired nature A. The formation of severe hepatitis, cirrhosis, and severe anemia. B. Consumption increases diffuse intravascular coagulation (DIC), thrombosis, major bleeding, use of snake venom preparations, and the like. C. Fibrinolysis into primary fibrinolysis, shock (shock), surgery (primary fibrinolysis), DIC (secondary fibrinolysis), moderate to low coagulation period. High results may be diseases: obstetric disseminated intravascular coagulation, thrombophlebitis, brain stem infarction, hand injury precautions (1) Blood collection should be smooth and should not be >45s, and the first 2ml should be discarded. The syringe should be silicided or plastic syringe. (2) Immediately after blood collection, inject into the test tube containing anticoagulant and mix it quickly in ice water. If it needs to be preserved, it is more suitable at -20 °C after adsorption treatment with bentonite. Inspection process (1) Take a blood sample tube with a 2ml scale, add 109mmol/L sodium citrate 0.2ml, then add about 2ml of venous blood to the mark and shake. The plasma was separated by centrifugation for 10 min. (2) Take 1 tube of 10×100mm small tube and add 0.2ml of plasma, then add 0.2ml of 36mmol/L calcium chloride solution or 0.2ml of thrombin, mix and set in a water bath at 37°C for 1h. (3) Lightly shake the small test tube to loosen the fibrin clot. Insert a small glass rod along the wall of the tube to gently press the fibrin clot, slowly release the fibrin, and finally roll the fibrin onto the small glass rod. (4) Wash the fibrin piece 4 times with water. (5) Take 2 tubes of 15×150mm and write the measuring tube (U) and the blank tube (B). (6) Put the fibrin into the U tube, add 0.2 ml of 2.5 mol/L sodium hydroxide to each of the U and B tubes, and then heat the U tube in a boiling water bath at 100 ° C for 5 min until the fibrin is completely dissolved. (7) Add about 5 ml of water and 1.0 ml of phenol reagent to each of the 2 tubes, add water to 10 ml each, and finally add 3 ml of each of 1.9 mol/L sodium carbonate solution. After standing at room temperature for 30 min, the B tube was used as a blank tube, and the absorbance of the U tube was read at a wavelength of 580 to 640 nm, and the standard curve of the pre-painted standard was used to obtain the plasma fibrinogen concentration result. Not suitable for the crowd Hemophilia and diffuse intravascular coagulation. Adverse reactions and risks Subcutaneous hemorrhage: subcutaneous hemorrhage due to less than 5 minutes of compression time or blood draw technique.

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