Thymic acid turbidity test

Determination of plasma protein levels and analysis of its components is one of the important tests for liver disease. The assays in this regard have been discussed in detail in other chapters. This section focuses on some simple serum colloidal stability tests related to protein metabolism. The principles of various serum colloid stability tests are basically the same, but the reagents used are different, and the effects of promoting and inhibiting flocculation are different. Currently, TTT tests are commonly used in clinical practice. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Serum should be fresh, and blood should be taken on an empty stomach in the morning. Normal value 0 to 6u. Clinical significance Various types of serum colloid stability tests may have different positive rates in different liver diseases or different periods of the same disease, each with certain clinical significance. 1. Diagnosis of acute hepatitis In acute hepatitis and acute liver damage, all kinds of serum flocculation and turbidity tests can be positive, and the total positive rate is about 80% to 90%. TTT began to rise in the first week of onset, peaked at two weeks, and then gradually decreased to normal. These changes can occur in the early stage of jaundice, so it has early diagnostic significance for acute hepatitis, but it is not as sensitive as transaminase, and the positive rate is lower than that of transaminase. The positive period is longer than the transaminase. This is because the protein has to be renewed for a certain period of time. For example, the half-life of albumin is 10 to 36 days. Therefore, the protein changes caused by liver diseases often take a certain period of time to manifest. In cases without jaundice hepatitis, serum colloid stability tests are often used as sensitive indicators for diagnosis. For example, the acute hepatitis flocculation test continues to be positive, suggesting that liver disease has a tendency to become chronic. 2. Judgment of hepatitis and cirrhosis In acute hepatitis, the change of TTT is parallel with the serum lipid rise and fall, but as the lesion is prolonged and chronic, it is positively correlated with serum gamma globulin, so TTT is positive. The reflection of chronic and chronic liver disease. 3, the identification of obstructive jaundice and hepatitis When obstructive jaundice patients have no hepatocyte damage, all kinds of serum flocculation test is mostly negative, and different from acute liver parenchymal damage, for the identification of jaundice type has a certain value. However, in patients with long-term obstructive jaundice or co-infection, liver parenchyma is often damaged. This test can also be positive, but it is generally seen in the middle and late stages of the disease. In cases of long-term jaundice, such as serum flocculation test continues to be negative, the possibility of congenital jaundice should be considered. 4, severe pregnancy and obstruction and pregnancy with hepatitis, pregnancy, mild bilirubinemia and elevated transaminase, but serum smear test is almost normal; on the contrary, pregnancy with hepatitis is 90 The serum flocculation test of all the above cases was positive. 5, when fatty liver, TTT often rises. Some people measured the TTT/ZnTT ​​ratio and found that it is higher than chronic hepatitis in fatty liver. It is considered that this ratio change can be used as a reference for differential diagnosis. In metastatic liver cancer, the TTT value is lower than that of primary liver cancer. Therefore, for patients with liver cancer, patients with low values ​​should consider metastatic liver cancer first, and those with high value should give priority to primary liver cancer, but only The reference value should also be based on clinical. In the case of schistosomiasis cirrhosis, serum flocculation tests are often positive. 6, extrahepatic disease serum colloid stability test is not specific to liver disease, in other diseases that can occur albumin reduction, globulin (gamma or beta globulin) or lipidoids such as malaria, connective tissue disease, kala-azar In the case of subacute bacterial endocarditis and multiple myeloma, it may also be positive. Precautions TTT is a very simple test, but the access between the laboratories is often not small. There are many factors contributing to this difference; first, although they are also called TTT tests, each laboratory is not the same in reagent preparation, standard curve drawing or standard tube preparation. Inspection process Take the same tube as the standard turbidity tube, add 0.05 ml of fresh serum, add 3 ml of thymol barbiturin buffer, mix, let stand for 30 min at room temperature, and then mix and invert several times. When the visual turbidity is cloudy, the light can be turned back, and the turbidity tube is lined with a word white paper to identify the degree of clarity of the writing to determine the turbidity unit. The unit number of the standard turbidiary tube which is the same as the measurement tube turbidity is the unit number of the measured serum. When turbidity is measured by a spectrophotometer, the "0" point is corrected with phenolic barbituration buffer, the wavelength is 650 nm, the absorbance of the measuring tube is read, and the standard curve is used to determine the turbidity unit. Not suitable for the crowd no. Adverse reactions and risks no.

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