Lysozyme

Lysozyme is a component of the normal immune defense mechanism and has the function of dissolving bacterial cell walls. In the human body, it is present in neutrophils, monocytes and macrophages; it is also present in mucosal secretions and is one of the surface defense factors. Normal people have no lysozyme in their urine. The lysozyme activity values ​​in serum or body fluids of some patients have significant differences, so the determination of lysozyme activity has received increasing clinical attention. Common methods are agar plate method and turbidimetry. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Acute lymphoblastic, acute erythroleukemia decreased or normal. Normal value: Lysozyme (agar plate method): 5-30mg/L Lysozyme (turbidimetric assay): 9.6-14 mg/L Above normal: It has certain significance for the identification of various types of acute leukemia, and the serum and serotonin lysozyme are elevated. negative: Positive: Reminder: The specimen should be fresh, and the lysozyme standard solution should be accurately prepared at the time of use, and the lysozyme activity in the sample should be determined. Normal value Serum 5-30 mg/L (agar plate method); 9.6-14 mg/L (turbidimetric assay). Cerebrospinal fluid 0 mg / L (agar plate method). Saliva 30 ~ 70mg / L (turbidimetric assay). Urine 0 mg / L (agar plate method); 1 ~ 3 mg / L (turbidimetric assay). Because the method and experimental conditions are different, the results are different, so each laboratory should establish its own normal value standard. Clinical significance Serum lysozyme assay has certain significance for the identification of various types of acute leukemia. The acute granule and acute single serum lysozyme are elevated; while acute lymphoblastic and acute erythroleukemia are decreased or normal; lysozyme level can be restored after chemotherapy has been relieved. Serum lysozyme assay can be used as a useful indicator to determine the activity of localized colitis and to help determine the severity of the clinical course and its response to treatment. The cause of increased urinary lysozyme content is 1 renal tubular damage; 2 high lysozymeemia; 3 renal tissue destruction. Clinically, urine lysozyme is mainly used as an indicator of renal tubular damage. Renal tubular damage of various reasons can cause an increase in urinary lysozyme content. Regular renal examination of lysozyme activity is necessary for kidney transplant patients. If the transplanted kidney is well received, the lysozyme activity returns to normal within 7 days; if excessive lysozyme persists in the urine, it must be suspected that rejection occurs. The content of lysozyme in cerebrospinal fluid (CSF) in patients with bacterial meningitis is much higher than that in patients with viral meningitis. Therefore, the determination of lysozyme is important for the identification of the two. Also. CSF lysozyme assay has certain diagnostic value for primary or secondary tumors of the central nervous system. The lysozyme content in sputum of patients with chronic bronchitis decreased; the lysozyme activity in serum or urine of patients with severe tuberculosis and urinary tract infections was significantly increased. In addition, the determination of lysozyme content can also be used as an indicator of the activity of localized enteritis. It also helps to assess the severity of the clinical process and the treatment response. High results may be diseases: urinary tract infection in the elderly, acute non-lymphocytic leukemia in the elderly The literature on the shelf life of the specimens and the storage time of the lysozyme standard solution are mixed. Generally speaking, the specimen should be fresh, and the lysozyme standard solution should be accurately prepared at the time of use, and the lysozyme activity in the sample should be determined. Lysozyme immunoassays have been established using anti-human lysozyme antibodies. From the enzyme activity to the enzyme content, it is preliminarily considered to be specific, sensitive and accurate. Inspection process It was determined by agar plate method and turbidimetry. Not suitable for the crowd no. Adverse reactions and risks no.

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