Esculin hydrolysis test

The esculetin hydrolysis test is based on the decomposition of esculin into glucose and escin by some bacteria, and the eschar and the ferrous iron of iron citrate in the medium are reacted to form a black compound. A method of bacterial identification based on the principle of black. Mainly used for the identification of group D streptococcus and other streptococci, the former is positive, the latter is negative. It can also be used for the identification of Gram-negative bacilli and anaerobic bacteria. Basic Information Specialist classification: Infectious disease inspection and classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a state of dynamic balance and health. Clinical significance Mainly used for the identification of group D streptococcus and other streptococci, the former is positive, the latter is negative. It can also be used for the identification of Gram-negative bacilli and anaerobic bacteria. Abnormal result 1. Streptococcus caused by streptococcus caused by various purulent inflammation, scarlet fever, rheumatic fever and other diseases. Group A Streptococcus caused by purulent infection tonsillitis, erysipelas, cellulitis, lymphangitis, sepsis; scarlet fever; rheumatic fever, streptococcal infection after glomerulonephritis. Group B Streptococcus conditional pathogens are the main pathogens of neonatal sepsis and meningitis. Streptococcus pneumonia causes diseases such as lobar pneumonia. A streptococcus conditional pathogen causes subacute bacterial endocarditis under certain conditions. Streptococcus mutans is closely related to dental caries 2. Infection symptoms caused by certain Gram-negative bacilli and anaerobic bacteria. The population to be examined is a patient with symptoms of infection caused by Streptococcus, certain Gram-negative bacilli, and anaerobic bacteria. Positive results may be diseases: scarlet fever, sepsis, suppurative meningitis considerations Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process Medium: esculin medium. Method: The test species were inoculated on the esculin medium and cultured overnight. The positive medium was brownish black, and the ones that were not discolored were negative. Not suitable for the crowd No. Adverse reactions and risks no.

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