Urinary Free Cortisol (UFC)

Urinary free cortisol is filtered from the free cortisol in the blood through the glomerulus, so the amount of base is directly proportional to the truly bioactive free cortisol in the plasma. Determination of urinary free cortisol can effectively and correctly reflect the state of adrenal function. Reduce adrenal insufficiency, Addison's disease, Simon's disease (Sinmond's disease), Xihan's disease (Sheehan's disease), long-term use of steroids. Basic Information Specialist classification: urinary examination classification: urine / kidney function test Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Adrenal insufficiency, Addison's disease, Simon's disease (Sinmond's disease), Xihan's disease (Sheehan's disease), long-term use of steroids. Normal value: Urinary free cortisol: 27.6-276.0nmol/24h Above normal: Increased is a reliable indicator for the diagnosis of Cushing's syndrome. Also seen in hypercortisolism, anterior pituitary hyperfunction. negative: Positive: Tips: Add 3H to each tube or the standard must be blown dry, then add the antibody, because 3H or standard is ethanol, otherwise it will damage the antibody. Normal value The normal value is 27.6 to 276.0 nmol/24 h. (Note the specific reference value depends on each laboratory.) Clinical significance Reduce adrenal insufficiency, Addison's disease, Simon's disease (Sinmond's disease), Xihan's disease (Sheehan's disease), long-term use of steroids. Increased is a reliable indicator for the diagnosis of Cushing's syndrome. Also seen in hypercortisolism, anterior pituitary hyperfunction. Normally and effectively exclude hypercortisolemia. In particular, Cushing's syndrome is caused by a variety of diseases caused by excessive secretion of glucocorticoids (mainly cortisol) from the adrenal gland. Low results may be diseases: high adrenal insufficiency may result in diseases: Cushing's syndrome considerations 1. Add 3H to each tube or the standard must be blown dry, then add the antibody, because 3H or standard is ethanol, otherwise it will damage the antibody. 2, the amount of 3H must be determined according to the experimental conditions (this article adds 7000 cpm), the less the amount of 3H antigen added in the corresponding range, the higher the sensitivity. Inspection process 1. Determination of urine sample: Take one ten thousandth of 24h mixed urine, dilute to 0.5ml with distilled water, add 10ml of dichloromethane and 0.2ml of 0.1mol/L NaOH in a 10ml extraction tube, extract for 5min, and discard the static piece. Remove the upper layer of liquid, wash the lower layer of dichloromethane extract twice with water, each time 1ml, shake for 1min, centrifuge 1500r/min for 3min, discard the water layer, take 1ml of dichloromethane extract in duplicate, 45 °C Compressed air was blown dry, add 3H-cortisol 7000 cpm/10 μl, blow dry at 45 ° C, add 0.2 ml of antibody application solution (1:3800 diluted with 1g/L GPS), shake and set at 4 ° C overnight, the following steps are the same as standard tube . 2, standard curve production: 0, 0.1, 0.3, 0.6, 1.0, 2.0, 4.0 ng (resection of each point), each tube plus 3H-cortisol 10ml, blow dry, add 0.2ml per antibody, take four Add 10 ml of 3H cortisol to each tube, add 1 g/L GPS0.7 and 0.2 ml, respectively, two tubes for the total pulse measuring tube, and the other two tubes for non-specific determination (BN%), shake well, and let 4 °C overnight. On the next day, add 5g/L GPS 0.1ml to each tube and shake it, then add 2.5g/LDCC0.5ml (the total tube T is not added). The free and conjugate were separated; shaken at 4 ° C for 10 min, centrifuged at 3000 r / min for 10 min to absorb 0.4 ml of the supernatant, added to 8 ml of scintillation fluid, mixed for 1 min, protected from light for 4 h, and measured with a liquid scintillation counter. Not suitable for the crowd Menstrual women. Adverse reactions and risks no.

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