Urinary alkaline phosphatase (UALP)

Alkaline phosphatase monophosphatase is widely present in various tissues of the human body. Determination of urinary alkaline phosphatase has a certain value for kidney disease, and when the renal parenchyma is damaged, the measurement result is increased. Basic Information Specialist classification: urinary examination classification: urine / kidney function test Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: No clinical significance. Normal value: Urinary alkaline phosphatase: 0.7-10.1 U/g·Cr Above normal: Common in glomerular filtration dysfunction, renal tubular epithelial cell necrosis (such as acute tubular necrosis), acute tubular nephritis, lupus nephritis, pyelonephritis, renal transplant rejection, urinary tumor, diabetic nephropathy, renal infarction Chronic nephritis. It can be used as an early diagnostic indicator of drug-induced renal injury. negative: Positive: Tips: Avoid the decomposition of phenyldiphenyl phosphate during storage. The distilled water should be boiled and disinfected every time. After cooling, add chloroform, store in the refrigerator, and pour out the required amount before use. The excess reagent can not be poured back into the bottle. Can be stored for 1 month. The order of adding reagents cannot be reversed. Normal value 0.7 to 10.1 U/g·Cr. Clinical significance Elevation: common in glomerular filtration dysfunction, renal tubular epithelial cell necrosis (such as acute tubular necrosis), acute tubular nephritis, lupus nephritis, pyelonephritis, renal transplant rejection, urinary tumor, diabetic nephropathy , kidney infarction, chronic nephritis. It can be used as an early diagnostic indicator of drug-induced renal injury. High results may be diseases: acute tubular necrosis, lupus nephritis precautions 1. Avoid disintegration during the preservation process of phenyl phosphate. The distilled water of each preparation reagent should be boiled and disinfected. After cooling, add chloroform, store in the refrigerator, and pour out the required amount before use. The excess reagent can not be poured back into the bottle. Can be stored for 1 month. 2. The order of adding reagents cannot be reversed. 3. The reagent blank is used instead of the serum control tube. The reagent blank tube can check whether the substrate is decomposed. When the absorbance is red, the substrate should not be used. When the serum is severely hemolyzed and jaundic, a serum control can be used. Inspection process 1. Colorimetric method: Mix well, adjust the absorbance of each tube at 510 nm, and record the absorbance of each tube to determine the difference between the absorbance of the tube and the control tube, and check the standard curve. 2, continuous monitoring method: each laboratory can be based on the analyzer model and operating instructions, the main parameters: incubation time 30s, continuous monitoring time 60s, wavelength 405nm, liquid absorption 500μl, temperature 37 ° C, specimen and reagent volume Ratio 1:50, coefficient 2757. Not suitable for the crowd no. Adverse reactions and risks no.

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