β-galactosidase

GAL is a hydrolase in cell lysosomes and is high in renal proximal tubular epithelial cells. GAL activity in the urine can reflect the renal parenchyma, especially the early damage of the renal tubule, and is measured together with urine N-acetyl-β-D-glucosaminidase (NAG) for urinary zymography analysis, which is helpful for disease course observation and prognosis. Evaluation. The diagnosis of human β-galactosidase deficiency disease (including prenatal diagnosis) and basic research in the field of genetics are also important indications. Basic Information Specialist classification: urinary examination classification: urine / kidney function test Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Rare. Normal value: GAL excretion rate in urine: 2.5-14.3 IU/gCr Above normal: Renal tubular damage. negative: Positive: Tips: According to the nutritional status of the whole body, milk, eggs, fruits, soy milk, etc. are given in the meal. Normal value The serum is 0.2-0.4 U/L, and the GAL discharge rate in urine is in the range of 2.5 to 14.3 IU/gCr. Clinical significance Urinary GAL and NAG are both lysosomal enzymes, mainly derived from renal tubular epithelial cells, and thus are more sensitive to renal tubular damage and acquired diseases. GAL and NAG excretion rate curves at different stages of renal parenchymal injury There are certain differences, and the two are used for zymography analysis, which will be helpful for clinical diagnosis and prognosis evaluation. Precautions 1. The linear range of the method can reach 220 IU/L. 2. The pH of the buffer has a significant influence on the extinction coefficient, and the pH requirement is accurately corrected to the specified value. 3. The rest can be found in the section "N-Acetyl-β-D-glucosaminidase". 4. For applications in the field of genetics, please refer to the monographs and literature. Inspection process Manual rate method: 100 μl of urine sample, 1.0 ml of substrate solution was added, and the initial absorbance A1 was measured after mixing, and the stopwatch was started immediately. The temperature was kept at 37 ° C for 10 min, and the second absorbance A2 was measured immediately. The wavelength was 400 to 405 nm, and the absorbance variable of 1 min was calculated. Not suitable for the crowd no. Adverse reactions and risks no.

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