β-carotene
Carotene has a chemical structure similar to that of vitamin A, and can be converted into vitamin A in the body, so it is also called vitamin A. The average absorption rate of β-carotene in the human body is 1/3 of the intake, and the conversion rate of conversion to vitamin A in the body is 1/2 of the absorption. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Cirrhosis of the liver. Normal value: --carotene: 0.93-3.7μmol/L Above normal: Mucinous edema, diabetes, chronic nephritis. A large intake of carotene, serum β-carotene up to 9.3μmol / L (500μg / dl), called caroteneemia, can disappear after a few weeks. negative: Positive: Tips: According to the nutritional status of the whole body, milk, eggs, fruits, soy milk, etc. are given in the meal. Normal value 0.93 to 3.7 μmol/L (50 to 200 μg/dl). Clinical significance 1, increased mucinous edema, diabetes, chronic nephritis. A large intake of carotene, serum β-carotene up to 9.3μmol / L (500μg / dl), called caroteneemia, can disappear after a few weeks. 2, reduce cirrhosis. Low results may be diseases: high results of systemic mucinous edema may be disease: cirrhosis precautions It is not advisable to eat foods rich in beta carotene before blood draw, such as green or yellow vegetables, such as spinach, grass head, pea sprouts, leeks, red sweet potatoes, carrots, pumpkins, etc. Inspection process 1. Pipette 3 ml of serum into a 25 ml glass stopper grind cone, add 3 ml of absolute ethanol, and rotate while mixing. Add 6 ml of petroleum ether, cover with a glass stopper, and mix thoroughly for about several minutes. 2. Transfer to a 15 ml stoppered centrifuge tube, stopper, and centrifuge at low speed (about 900 g) for about 1 to 2 minutes. 3. All the petroleum ether layers are sucked out and placed in a test tube with a stopper, to be tested. 4. Using 450nm wavelength, 1cm cuvette, correct the absorbance to 0 points with petroleum ether, read the absorbance of the above extract, check the standard curve, and find the content of serum β-carotene (retain the colorimetric liquid for vitamin A). Determination). Not suitable for the crowd no. Adverse reactions and risks no.
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