polymerase chain reaction

The polymerase chain reaction is a method for rapidly amplifying a specific gene or DNA sequence in vitro, and is also referred to as an in vitro amplification method of a gene. PCR technology is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. Basic Information Specialist Category: Infectious Diseases Inspection Category: Genetic Testing (DNA) Applicable gender: whether men and women apply fasting: not fasting Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value Molecular hybridization was negative. Clinical significance PCR can rapidly and specifically amplify any known gene or DNA fragment of interest, and can easily amplify the target gene in the starting DNA mixture at the picogram (pg) level to a nanogram, microgram, and milligram-specific DNA fragment. Therefore, PCR technology has been rapidly and widely used in various fields of molecular biology. Abnormal results abnormalities caused by various diseases, such as syphilis: 1 stage syphilis. That is, hard chancre, with an incubation period of 2 to 4 weeks, a dark red hard mass, a shallow ulcer, a cartilage-like hardness, and a swollen peripheral lymph node. 2 second phase syphilis. After 1 to 2 months of primary syphilis, sympathetic rash, rash, pimples, pustular rash, etc. occur in the skin and mucous membranes of the whole body. Mucosal plaques, flat wet phlegm can occur in the mucosa, and the infection is strong. 3 third-phase syphilis. Occurred 2 to 3 years or even 10 years after infection, the skin is a gum-like swelling, and may also involve bone, joint, heart, blood vessels, manifesting aortic inflammation, aortic insufficiency and aortic aneurysm, etc., invading the nerve Spinal cord paralysis, general paralysis (paralytic dementia) and so on. The person in need of examination is suspected of a specific disease patient and undergoes a molecular specific examination. Precautions Preparation before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Patients with a history of fainting should explain in advance and we will make special arrangements. Requirements for inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Not suitable for people: There is no inappropriate crowd. Inspection process Syphilis is a chronic systemic infectious disease common in sexually transmitted diseases. The body is infected with Treponema pallidum (TP, scientific name Treponema). Two major antibodies are produced in the serum: one is a non-specific antibody, that is, a reactive hormone; the other is a specific antibody. Currently commonly used tests are: (1) inactivated serum responsiveness test (USR). This test is generally used for diagnostic screening and epidemiological investigation of melanin. (2) Rapid plasma responsiveness test (RPR). This test can also be used as an auxiliary diagnosis of melan. (3) Fluorescent Treponema Hemagglutination Test (FTA-ABS). This test detects syphilis-specific antibodies. (4) Treponema antibody hemagglutination test (TPHA). This test detects a melamine-specific antibody and is a melamine diagnostic test. Not suitable for the crowd Taboo population: There are no special taboos. Adverse reactions and risks This test generally does not cause special complications and hazards.

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